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The Nucleus

Overview of attention for book
Cover of 'The Nucleus'

Table of Contents

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    Book Overview
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    Chapter 1 Cell type-specific affinity purification of nuclei for chromatin profiling in whole animals.
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    Chapter 2 Lysis Gradient Centrifugation: A Flexible Method for the Isolation of Nuclei from Primary Cells
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    Chapter 3 Isolation of Nuclei in Media Containing an Inert Polymer to Mimic the Crowded Cytoplasm
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    Chapter 4 A New Rapid Method for Isolating Nucleoli
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    Chapter 5 Sequential recovery of macromolecular components of the nucleolus.
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    Chapter 6 Au Nanoinjectors for Electrotriggered Gene Delivery into the Cell Nucleus
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    Chapter 7 Improving Chromatin Immunoprecipitation (ChIP) by Suppression of Method-Induced DNA-Damage Signaling
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    Chapter 8 Purification of specific chromatin Loci for proteomic analysis.
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    Chapter 9 Chromatin structure analysis of single gene molecules by psoralen cross-linking and electron microscopy.
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    Chapter 10 Purification of Proteins on Newly Synthesized DNA Using iPOND.
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    Chapter 11 Applying the ribopuromycylation method to detect nuclear translation.
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    Chapter 12 Targeted Nano Analysis of Water and Ions in the Nucleus Using Cryo-Correlative Microscopy
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    Chapter 13 A Redox-Sensitive Yellow Fluorescent Protein Sensor for Monitoring Nuclear Glutathione Redox Dynamics
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    Chapter 14 Determination of the Dissociation Constant of the NFκB p50/p65 Heterodimer in Living Cells Using Fluorescence Cross-Correlation Spectroscopy
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    Chapter 15 Imaging and quantification of amyloid fibrillation in the cell nucleus.
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    Chapter 16 Analysis of Nuclear Organization with TANGO, Software for High-Throughput Quantitative Analysis of 3D Fluorescence Microscopy Images
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    Chapter 17 Quantitative Analysis of Chromosome Localization in the Nucleus
Attention for Chapter 10: Purification of Proteins on Newly Synthesized DNA Using iPOND.
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About this Attention Score

  • In the top 25% of all research outputs scored by Altmetric
  • High Attention Score compared to outputs of the same age (84th percentile)
  • High Attention Score compared to outputs of the same age and source (95th percentile)

Mentioned by

1 blog
2 tweeters


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Chapter title
Purification of Proteins on Newly Synthesized DNA Using iPOND.
Chapter number 10
Book title
The Nucleus
Published in
Methods in molecular biology, January 2015
DOI 10.1007/978-1-4939-1680-1_10
Pubmed ID
Book ISBNs
978-1-4939-1679-5, 978-1-4939-1680-1

Huzefa Dungrawala, David Cortez, Dungrawala H, Cortez D


The replisome is a large protein machine containing multiple enzymatic activities needed to complete DNA replication. In addition to helicase and polymerases needed for copying the DNA, the replisome also contains proteins like DNA methyltransferases, histone chaperones, and chromatin modifying enzymes to couple DNA replication with chromatin deposition and establishment of the epigenetic code. In addition, since template DNA strands often contain DNA damage or other roadblocks to the replication machinery, replication stress response proteins associate with the replisome to stabilize, repair, and restart stalled replication forks. Hundreds of proteins are needed to accomplish these tasks. Identifying these proteins, monitoring their posttranslational modifications, and understanding how their activities are coordinated is essential to understand how the genome and epigenome are duplicated rapidly, completely, and accurately every cell division cycle. Here we describe an updated iPOND (isolation of proteins on nascent DNA) method to facilitate these analyses.

Twitter Demographics

The data shown below were collected from the profiles of 2 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 74 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 74 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 22 30%
Researcher 22 30%
Student > Doctoral Student 7 9%
Student > Bachelor 5 7%
Student > Master 4 5%
Other 5 7%
Unknown 9 12%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 37 50%
Agricultural and Biological Sciences 20 27%
Medicine and Dentistry 3 4%
Chemistry 2 3%
Immunology and Microbiology 1 1%
Other 1 1%
Unknown 10 14%

Attention Score in Context

This research output has an Altmetric Attention Score of 8. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 19 January 2018.
All research outputs
of 12,388,051 outputs
Outputs from Methods in molecular biology
of 8,329 outputs
Outputs of similar age
of 216,911 outputs
Outputs of similar age from Methods in molecular biology
of 73 outputs
Altmetric has tracked 12,388,051 research outputs across all sources so far. Compared to these this one has done well and is in the 85th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 8,329 research outputs from this source. They receive a mean Attention Score of 2.1. This one has done particularly well, scoring higher than 93% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 216,911 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 84% of its contemporaries.
We're also able to compare this research output to 73 others from the same source and published within six weeks on either side of this one. This one has done particularly well, scoring higher than 95% of its contemporaries.