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The Nucleus

Overview of attention for book
Cover of 'The Nucleus'

Table of Contents

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    Book Overview
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    Chapter 1 Cell type-specific affinity purification of nuclei for chromatin profiling in whole animals.
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    Chapter 2 Lysis Gradient Centrifugation: A Flexible Method for the Isolation of Nuclei from Primary Cells
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    Chapter 3 Isolation of Nuclei in Media Containing an Inert Polymer to Mimic the Crowded Cytoplasm
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    Chapter 4 A New Rapid Method for Isolating Nucleoli
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    Chapter 5 Sequential recovery of macromolecular components of the nucleolus.
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    Chapter 6 Au Nanoinjectors for Electrotriggered Gene Delivery into the Cell Nucleus
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    Chapter 7 Improving Chromatin Immunoprecipitation (ChIP) by Suppression of Method-Induced DNA-Damage Signaling
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    Chapter 8 Purification of specific chromatin Loci for proteomic analysis.
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    Chapter 9 Chromatin structure analysis of single gene molecules by psoralen cross-linking and electron microscopy.
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    Chapter 10 Purification of Proteins on Newly Synthesized DNA Using iPOND.
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    Chapter 11 Applying the ribopuromycylation method to detect nuclear translation.
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    Chapter 12 Targeted Nano Analysis of Water and Ions in the Nucleus Using Cryo-Correlative Microscopy
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    Chapter 13 A Redox-Sensitive Yellow Fluorescent Protein Sensor for Monitoring Nuclear Glutathione Redox Dynamics
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    Chapter 14 Determination of the Dissociation Constant of the NFκB p50/p65 Heterodimer in Living Cells Using Fluorescence Cross-Correlation Spectroscopy
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    Chapter 15 Imaging and quantification of amyloid fibrillation in the cell nucleus.
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    Chapter 16 Analysis of Nuclear Organization with TANGO, Software for High-Throughput Quantitative Analysis of 3D Fluorescence Microscopy Images
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    Chapter 17 Quantitative Analysis of Chromosome Localization in the Nucleus
Attention for Chapter 1: Cell type-specific affinity purification of nuclei for chromatin profiling in whole animals.
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Chapter title
Cell type-specific affinity purification of nuclei for chromatin profiling in whole animals.
Chapter number 1
Book title
The Nucleus
Published in
Methods in molecular biology, January 2015
DOI 10.1007/978-1-4939-1680-1_1
Pubmed ID
25311117
Book ISBNs
978-1-4939-1679-5, 978-1-4939-1680-1
Authors

Florian A Steiner, Steven Henikoff, Florian A. Steiner, Steiner, Florian A., Henikoff, Steven

Abstract

Analyzing cell differentiation during development in a complex organism requires the analysis of expression and chromatin profiles in individual cell types. Our laboratory has developed a simple and generally applicable strategy to purify specific cell types from whole organisms for simultaneous analysis of chromatin and expression. The method, termed INTACT for Isolation of Nuclei TAgged in specific Cell Types, depends on the expression of an affinity-tagged nuclear envelope protein in the cell type of interest. These nuclei can be affinity-purified from the total pool of nuclei and used as a source for RNA and chromatin. The method serves as a simple and scalable alternative to FACS sorting or laser capture microscopy to circumvent the need for expensive equipment and specialized skills. This chapter provides detailed protocols for the cell-type specific purification of nuclei from Caenorhabditis elegans.

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X Demographics

The data shown below were collected from the profiles of 2 X users who shared this research output. Click here to find out more about how the information was compiled.
 Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 21 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Netherlands 1 5%
Unknown 20 95%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 5 24%
Student > Bachelor 4 19%
Researcher 4 19%
Professor > Associate Professor 2 10%
Student > Master 1 5%
Other 3 14%
Unknown 2 10%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 9 43%
Agricultural and Biological Sciences 7 33%
Medicine and Dentistry 2 10%
Neuroscience 1 5%
Unknown 2 10%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 14 June 2015.
All research outputs
#18,380,628
of 22,766,595 outputs
Outputs from Methods in molecular biology
#7,871
of 13,090 outputs
Outputs of similar age
#255,649
of 352,895 outputs
Outputs of similar age from Methods in molecular biology
#479
of 996 outputs
Altmetric has tracked 22,766,595 research outputs across all sources so far. This one is in the 11th percentile – i.e., 11% of other outputs scored the same or lower than it.
So far Altmetric has tracked 13,090 research outputs from this source. They receive a mean Attention Score of 3.3. This one is in the 24th percentile – i.e., 24% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 352,895 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 16th percentile – i.e., 16% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 996 others from the same source and published within six weeks on either side of this one. This one is in the 36th percentile – i.e., 36% of its contemporaries scored the same or lower than it.

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