Report for: Peptide Nucleic Acids

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Chapter title	PNA Fluorescent In Situ Hybridization (FISH) for Rapid Microbiology and Cytogenetic Analysis
Chapter number	14
Book title	Peptide Nucleic Acids
Published in	Methods in molecular biology, January 2014
DOI	10.1007/978-1-62703-553-8_14
Pubmed ID	24297359
Book ISBNs	978-1-62703-552-1, 978-1-62703-553-8
Authors	Henrik Stender, Brett Williams, James Coull
Abstract	Hybridization-based assays for the detection of nucleic acids including in situ hybridization are increasingly being utilized in a wide variety of disciplines such as cytogenetics, microbiology, and histology. Generally in situ hybridization assays utilize either cloned genomic probes for the detection of DNA sequences or oligonucleotide probes for the detection of DNA or RNA sequences. Alternately, PNA probes are increasingly being utilized in a variety of in situ hybridization assays. The neutral backbone of the PNA molecule allows for the PNA probes to bind to DNA or RNA under low ionic strength conditions that will either disfavor reannealing of complimentary genomic sequences or are denaturing for RNA secondary structure but are favorable for PNA/DNA or PNA/RNA hybridization. For in situ hybridization assays these unique properties of PNA probes offer significant advantages that allow for the development of fast, simple, and robust assays (Figs. 14.1 and 14.2).

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Mendeley readers

The data shown below were compiled from readership statistics for 21 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country	Count	As %
United States	1	5%
Unknown	20	95%

Demographic breakdown

Readers by professional status	Count	As %
Other	2	10%
Student > Master	2	10%
Researcher	2	10%
Student > Ph. D. Student	2	10%
Student > Doctoral Student	1	5%
Other	4	19%
Unknown	8	38%

Readers by discipline	Count	As %
Agricultural and Biological Sciences	3	14%
Biochemistry, Genetics and Molecular Biology	2	10%
Medicine and Dentistry	2	10%
Engineering	2	10%
Social Sciences	1	5%
Other	3	14%
Unknown	8	38%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 26 October 2014.

All research outputs

#20,241,019

of 22,768,097 outputs

Outputs from Methods in molecular biology

#9,865

of 13,090 outputs

Outputs of similar age

#264,855

of 305,310 outputs

Outputs of similar age from Methods in molecular biology

#405

of 597 outputs

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So far Altmetric has tracked 13,090 research outputs from this source. They receive a mean Attention Score of 3.3. This one is in the 1st percentile – i.e., 1% of its peers scored the same or lower than it.

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Peptide Nucleic Acids

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Demographic breakdown

Attention Score in Context