Chapter title |
Evaluating the Role of Viral Proteins in HIV-Mediated Neurotoxicity Using Primary Human Neuronal Cultures.
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Chapter number | 25 |
Book title |
HIV Protocols
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Published in |
Methods in molecular biology, January 2016
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DOI | 10.1007/978-1-4939-3046-3_25 |
Pubmed ID | |
Book ISBNs |
978-1-4939-3045-6, 978-1-4939-3046-3
|
Authors |
Rao, Vasudev R, Eugenin, Eliseo A, Prasad, Vinayaka R, Vasudev R. Rao, Eliseo A. Eugenin, Vinayaka R. Prasad, Rao, Vasudev R., Eugenin, Eliseo A., Prasad, Vinayaka R. |
Abstract |
Despite the inability of HIV-1 to infect neurons, over half of the HIV-1-infected population in the USA suffers from neurocognitive dysfunction. HIV-infected immune cells in the periphery enter the central nervous system by causing a breach in the blood-brain barrier. The damage to the neurons is mediated by viral and host toxic products released by activated and infected immune and glial cells. To evaluate the toxicity of any viral isolate, viral protein, or host inflammatory protein, we describe a protocol to assess the neuronal apoptosis and synaptic compromise in primary cultures of human neurons and astrocytes. |
X Demographics
Geographical breakdown
Country | Count | As % |
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United States | 1 | 33% |
Unknown | 2 | 67% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 3 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 9 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 3 | 33% |
Professor | 1 | 11% |
Student > Doctoral Student | 1 | 11% |
Student > Master | 1 | 11% |
Unknown | 3 | 33% |
Readers by discipline | Count | As % |
---|---|---|
Environmental Science | 1 | 11% |
Pharmacology, Toxicology and Pharmaceutical Science | 1 | 11% |
Nursing and Health Professions | 1 | 11% |
Medicine and Dentistry | 1 | 11% |
Neuroscience | 1 | 11% |
Other | 0 | 0% |
Unknown | 4 | 44% |