| Chapter title |
Mammary Cancer Stem Cells Reinitiation Assessment at the Metastatic Niche: The Lung and Bone
|
|---|---|
| Chapter number | 13 |
| Book title |
Mammary Stem Cells
|
| Published in |
Methods in molecular biology, January 2015
|
| DOI | 10.1007/978-1-4939-2519-3_13 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-2518-6, 978-1-4939-2519-3
|
| Authors |
Marc Guiu, Enrique J. Arenas, Sylwia Gawrzak, Milica Pavlovic, Roger R. Gomis, Guiu, Marc, Arenas, Enrique J., Gawrzak, Sylwia, Pavlovic, Milica, Gomis, Roger R. |
| Abstract |
Mammary cancer stem cells (MCSC) have been operationally defined as cells that re-form secondary tumors upon transplantation into immunodeficient mice. Building on this observation, it has also been suggested that MCSCs are responsible for metastasis as well as evasion and resistance to therapeutic treatments. MCSC reinitiating potential is usually tested by implantation of limited amounts of cells orthotopically or subcutaneously, yet this poorly recapitulates the metastatic niche where truly metastatic reinitiation will occur. Herein, we describe the implantation of small amounts of MCSC selected populations in the bone (intra tibiae injection) and the lung (intra thoracic injection) to test for their metastatic reinitiation capabilities. |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 11 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 3 | 27% |
| Professor | 2 | 18% |
| Student > Doctoral Student | 2 | 18% |
| Student > Ph. D. Student | 1 | 9% |
| Unknown | 3 | 27% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 3 | 27% |
| Medicine and Dentistry | 2 | 18% |
| Agricultural and Biological Sciences | 2 | 18% |
| Pharmacology, Toxicology and Pharmaceutical Science | 1 | 9% |
| Unknown | 3 | 27% |