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Mendeley readers
| Chapter title |
FACS Sorting Mammary Stem Cells
|
|---|---|
| Chapter number | 3 |
| Book title |
Mammary Stem Cells
|
| Published in |
Methods in molecular biology, January 2015
|
| DOI | 10.1007/978-1-4939-2519-3_3 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-2518-6, 978-1-4939-2519-3
|
| Authors |
Oihana Iriondo, Miriam Rábano, María d. M. Vivanco |
| Abstract |
Fluorescent-activated cell sorting (FACS) represents one of the key techniques that have been used to isolate and characterize stem cells, including cells from the mammary gland. A combination of approaches, including recognition of cell surface antigens and different cellular activities, has facilitated the identification of stem cells from the healthy mammary gland and from breast tumors. In this chapter we describe the protocol to use FACS to separate breast cancer stem cells, but most of the general principles discussed could be applied to sort other types of cells. |
Mendeley readers
The data shown below were compiled from readership statistics for 10 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 10 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Master | 3 | 30% |
| Student > Ph. D. Student | 2 | 20% |
| Student > Bachelor | 2 | 20% |
| Student > Doctoral Student | 1 | 10% |
| Unknown | 2 | 20% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 3 | 30% |
| Medicine and Dentistry | 2 | 20% |
| Chemistry | 2 | 20% |
| Agricultural and Biological Sciences | 1 | 10% |
| Unknown | 2 | 20% |