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Mendeley readers
| Chapter title |
Selective Evaluation of Thioredoxin Reductase Enzymatic Activities
|
|---|---|
| Chapter number | 21 |
| Book title |
Selenoproteins
|
| Published in |
Methods in molecular biology, January 2018
|
| DOI | 10.1007/978-1-4939-7258-6_21 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-7257-9, 978-1-4939-7258-6
|
| Authors |
Elias S. J. Arnér, Arnér, Elias S. J. |
| Abstract |
Thioredoxin reductases are important oxidoreductases that keep the active site disulfide/dithiol motif of thioredoxins reduced using NADPH, thereby supporting many thioredoxin-dependent reductive pathways in cells. Mammalian thioredoxin reductases are selenoproteins that have several additional substrates beyond thioredoxins. This chapter first lists several different assays for measurement of thioredoxin reductase activities, before giving a protocol for a selective evaluation of these activities that can be used in either crude cell lysates as well as with purified enzymes. The same assay can also be easily adopted for the determination of thioredoxin activities. |
Mendeley readers
The data shown below were compiled from readership statistics for 11 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 11 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Professor | 2 | 18% |
| Unspecified | 1 | 9% |
| Student > Bachelor | 1 | 9% |
| Researcher | 1 | 9% |
| Unknown | 6 | 55% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 3 | 27% |
| Unspecified | 1 | 9% |
| Pharmacology, Toxicology and Pharmaceutical Science | 1 | 9% |
| Immunology and Microbiology | 1 | 9% |
| Unknown | 5 | 45% |