↓ Skip to main content

Selenoproteins

Overview of attention for book
Cover of 'Selenoproteins'

Table of Contents

  1. Altmetric Badge
    Book Overview
  2. Altmetric Badge
    Chapter 1 SECISearch3 and Seblastian: In-Silico Tools to Predict SECIS Elements and Selenoproteins
  3. Altmetric Badge
    Chapter 2 Selenoprofiles: A Computational Pipeline for Annotation of Selenoproteins
  4. Altmetric Badge
    Chapter 3 SelGenAmic: An Algorithm for Selenoprotein Gene Assembly
  5. Altmetric Badge
    Chapter 4 Selenocysteine tRNA[Ser]Sec, the Central Component of Selenoprotein Biosynthesis: Isolation, Identification, Modification, and Sequencing
  6. Altmetric Badge
    Chapter 5 Identification and Characterization of Proteins that Bind to Selenoprotein 3′ UTRs
  7. Altmetric Badge
    Chapter 6 Specific Chemical Approaches for Studying Mammalian Ribosomes Complexed with Ligands Involved in Selenoprotein Synthesis
  8. Altmetric Badge
    Chapter 7 In Vitro Translation Assays for Selenocysteine Insertion
  9. Altmetric Badge
    Chapter 8 Studying Selenoprotein mRNA Translation Using RNA-Seq and Ribosome Profiling
  10. Altmetric Badge
    Chapter 9 Modification of Selenoprotein mRNAs by Cap Tri-methylation
  11. Altmetric Badge
    Chapter 10 Total Selenium Quantification in Biological Samples by Inductively Coupled Plasma Mass Spectrometry (ICP-MS)
  12. Altmetric Badge
    Chapter 11 Quantification of SeMet and SeCys in Biological Fluids and Tissues by Liquid Chromatography Coupled to Inductively Coupled Plasma Mass Spectrometry (HPLC-ICP MS)
  13. Altmetric Badge
    Chapter 12 Simultaneous Speciation of Selenoproteins and Selenometabolites in Plasma and Serum
  14. Altmetric Badge
    Chapter 13 Radioactive 75 Se Labeling and Detection of Selenoproteins
  15. Altmetric Badge
    Chapter 14 Nonradioactive Isotopic Labeling and Tracing of Selenoproteins in Cultured Cell Lines
  16. Altmetric Badge
    Chapter 15 Detection of Selenoproteins by Laser Ablation Inductively Coupled Plasma Mass Spectrometry (LA-ICP MS) in Immobilized pH Gradient (IPG) Strips
  17. Altmetric Badge
    Chapter 16 Imaging of Selenium by Laser Ablation Inductively Coupled Plasma Mass Spectrometry (LA-ICP-MS) in 2-D Electrophoresis Gels and Biological Tissues
  18. Altmetric Badge
    Chapter 17 Overexpression of Recombinant Selenoproteins in E. coli
  19. Altmetric Badge
    Chapter 18 Preparation of Selenocysteine-Containing Forms of Human SELENOK and SELENOS
  20. Altmetric Badge
    Chapter 19 Selenocysteine-Mediated Expressed Protein Ligation of SELENOM
  21. Altmetric Badge
    Chapter 20 Monitoring of Methionine Sulfoxide Content and Methionine Sulfoxide Reductase Activity
  22. Altmetric Badge
    Chapter 21 Selective Evaluation of Thioredoxin Reductase Enzymatic Activities
  23. Altmetric Badge
    Chapter 22 Association of Single Nucleotide Polymorphisms in Selenoprotein Genes with Cancer Risk
  24. Altmetric Badge
    Chapter 23 Identification of Genetic Disorders Causing Disruption of Selenoprotein Biosynthesis
Attention for Chapter 12: Simultaneous Speciation of Selenoproteins and Selenometabolites in Plasma and Serum
Altmetric Badge

Readers on

mendeley
13 Mendeley
You are seeing a free-to-access but limited selection of the activity Altmetric has collected about this research output. Click here to find out more.
Chapter title
Simultaneous Speciation of Selenoproteins and Selenometabolites in Plasma and Serum
Chapter number 12
Book title
Selenoproteins
Published in
Methods in molecular biology, January 2018
DOI 10.1007/978-1-4939-7258-6_12
Pubmed ID
Book ISBNs
978-1-4939-7257-9, 978-1-4939-7258-6
Authors

Belén Callejón-Leblic, Gema Rodríguez-Moro, Tamara García-Barrera, José Luis Gómez-Ariza

Abstract

Selenium is an essential element incorporated to different proteins with important biological functions in connection to antioxidant activity, cancer-protective properties, neurodegenerative pathologies, and prevention of effects of diabetes, among others. In addition, selenoamino acids play a basic role in the global equilibrium of key selenium-biomolecules synthesis, including selenoprotein P, selenoalbumin, and glutathione peroxidase. Homeostasis of these selenium-containing biomolecules involves different organs in living organisms including human, and bloodstream is the connection fluid in this process. Therefore, it is very important to have an analytical methodology suitable for selenium proteins and metabolites speciation in serum and plasma samples. For this purpose, a simultaneous speciation method for Se-containing biomolecules in serum/plasma is described on the basis of in series three-dimensional chromatography: size exclusion, affinity, and anion exchange high performance liquid chromatography (3D/SE-AF-AEC-HPLC), using different columns of each type and hyphenation to inductively coupled plasma-(quadrupole) mass spectrometry (ICP-MS). The method allows the quantitative simultaneous analysis of selenoprotein P (SeP), extracellular glutathione peroxidase (eGPx), selenoalbumin (SeAlb), selenite, and selenate in serum (from human and mouse) using species-unspecific isotope dilution (SUID). In addition, a simplified two-dimensional approach (2D/SE-AF-HPLC-SUID-ICP-MS) is described when selenium metabolites are globally analyzed. The method provides detection limits in the range 0.2-1.3 ng of Se g(-1) and avoids typical interferences in this matrix from chloride and bromide with a chromatographic runtime less than 35 min.

Mendeley readers

The data shown below were compiled from readership statistics for 13 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 13 100%

Demographic breakdown

Readers by professional status Count As %
Professor > Associate Professor 4 31%
Student > Master 2 15%
Student > Doctoral Student 2 15%
Researcher 2 15%
Student > Ph. D. Student 1 8%
Other 0 0%
Unknown 2 15%
Readers by discipline Count As %
Chemistry 3 23%
Biochemistry, Genetics and Molecular Biology 2 15%
Agricultural and Biological Sciences 1 8%
Nursing and Health Professions 1 8%
Psychology 1 8%
Other 3 23%
Unknown 2 15%