Chapter title |
Imaging of Selenium by Laser Ablation Inductively Coupled Plasma Mass Spectrometry (LA-ICP-MS) in 2-D Electrophoresis Gels and Biological Tissues
|
---|---|
Chapter number | 16 |
Book title |
Selenoproteins
|
Published in |
Methods in molecular biology, January 2018
|
DOI | 10.1007/978-1-4939-7258-6_16 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7257-9, 978-1-4939-7258-6
|
Authors |
Elisa Castañeda Santa Cruz, J. Susanne Becker, J. Sabine Becker, Alessandra Sussulini, Cruz, Elisa Castañeda Santa, Susanne Becker, J., Sabine Becker, J., Sussulini, Alessandra |
Abstract |
Selenium and selenoproteins are important components of living organisms that play a role in different biological processes. Laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) is a powerful analytical technique that has been employed to obtain distribution maps of selenium in biological tissues in a direct manner, as well as in selenoproteins, previously separated by their molecular masses and isoelectric points using two-dimensional polyacrylamide gel electrophoresis (2-D PAGE). In this chapter, we present the protocols to perform LA-ICP-MS imaging experiments, allowing the distribution visualization and determination of selenium and/or selenoproteins in biological systems. |
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