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Selenoproteins

Overview of attention for book
Cover of 'Selenoproteins'

Table of Contents

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    Book Overview
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    Chapter 1 SECISearch3 and Seblastian: In-Silico Tools to Predict SECIS Elements and Selenoproteins
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    Chapter 2 Selenoprofiles: A Computational Pipeline for Annotation of Selenoproteins
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    Chapter 3 SelGenAmic: An Algorithm for Selenoprotein Gene Assembly
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    Chapter 4 Selenocysteine tRNA[Ser]Sec, the Central Component of Selenoprotein Biosynthesis: Isolation, Identification, Modification, and Sequencing
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    Chapter 5 Identification and Characterization of Proteins that Bind to Selenoprotein 3′ UTRs
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    Chapter 6 Specific Chemical Approaches for Studying Mammalian Ribosomes Complexed with Ligands Involved in Selenoprotein Synthesis
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    Chapter 7 In Vitro Translation Assays for Selenocysteine Insertion
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    Chapter 8 Studying Selenoprotein mRNA Translation Using RNA-Seq and Ribosome Profiling
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    Chapter 9 Modification of Selenoprotein mRNAs by Cap Tri-methylation
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    Chapter 10 Total Selenium Quantification in Biological Samples by Inductively Coupled Plasma Mass Spectrometry (ICP-MS)
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    Chapter 11 Quantification of SeMet and SeCys in Biological Fluids and Tissues by Liquid Chromatography Coupled to Inductively Coupled Plasma Mass Spectrometry (HPLC-ICP MS)
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    Chapter 12 Simultaneous Speciation of Selenoproteins and Selenometabolites in Plasma and Serum
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    Chapter 13 Radioactive 75 Se Labeling and Detection of Selenoproteins
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    Chapter 14 Nonradioactive Isotopic Labeling and Tracing of Selenoproteins in Cultured Cell Lines
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    Chapter 15 Detection of Selenoproteins by Laser Ablation Inductively Coupled Plasma Mass Spectrometry (LA-ICP MS) in Immobilized pH Gradient (IPG) Strips
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    Chapter 16 Imaging of Selenium by Laser Ablation Inductively Coupled Plasma Mass Spectrometry (LA-ICP-MS) in 2-D Electrophoresis Gels and Biological Tissues
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    Chapter 17 Overexpression of Recombinant Selenoproteins in E. coli
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    Chapter 18 Preparation of Selenocysteine-Containing Forms of Human SELENOK and SELENOS
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    Chapter 19 Selenocysteine-Mediated Expressed Protein Ligation of SELENOM
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    Chapter 20 Monitoring of Methionine Sulfoxide Content and Methionine Sulfoxide Reductase Activity
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    Chapter 21 Selective Evaluation of Thioredoxin Reductase Enzymatic Activities
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    Chapter 22 Association of Single Nucleotide Polymorphisms in Selenoprotein Genes with Cancer Risk
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    Chapter 23 Identification of Genetic Disorders Causing Disruption of Selenoprotein Biosynthesis
Attention for Chapter 16: Imaging of Selenium by Laser Ablation Inductively Coupled Plasma Mass Spectrometry (LA-ICP-MS) in 2-D Electrophoresis Gels and Biological Tissues
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Chapter title
Imaging of Selenium by Laser Ablation Inductively Coupled Plasma Mass Spectrometry (LA-ICP-MS) in 2-D Electrophoresis Gels and Biological Tissues
Chapter number 16
Book title
Selenoproteins
Published in
Methods in molecular biology, January 2018
DOI 10.1007/978-1-4939-7258-6_16
Pubmed ID
Book ISBNs
978-1-4939-7257-9, 978-1-4939-7258-6
Authors

Elisa Castañeda Santa Cruz, J. Susanne Becker, J. Sabine Becker, Alessandra Sussulini, Cruz, Elisa Castañeda Santa, Susanne Becker, J., Sabine Becker, J., Sussulini, Alessandra

Abstract

Selenium and selenoproteins are important components of living organisms that play a role in different biological processes. Laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) is a powerful analytical technique that has been employed to obtain distribution maps of selenium in biological tissues in a direct manner, as well as in selenoproteins, previously separated by their molecular masses and isoelectric points using two-dimensional polyacrylamide gel electrophoresis (2-D PAGE). In this chapter, we present the protocols to perform LA-ICP-MS imaging experiments, allowing the distribution visualization and determination of selenium and/or selenoproteins in biological systems.

Twitter Demographics

The data shown below were collected from the profile of 1 tweeter who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 12 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 12 100%

Demographic breakdown

Readers by professional status Count As %
Professor 3 25%
Student > Postgraduate 2 17%
Student > Ph. D. Student 1 8%
Student > Bachelor 1 8%
Student > Master 1 8%
Other 2 17%
Unknown 2 17%
Readers by discipline Count As %
Chemistry 7 58%
Environmental Science 1 8%
Neuroscience 1 8%
Medicine and Dentistry 1 8%
Unknown 2 17%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 27 July 2020.
All research outputs
#10,261,208
of 16,088,768 outputs
Outputs from Methods in molecular biology
#3,270
of 9,320 outputs
Outputs of similar age
#170,379
of 282,618 outputs
Outputs of similar age from Methods in molecular biology
#6
of 7 outputs
Altmetric has tracked 16,088,768 research outputs across all sources so far. This one is in the 23rd percentile – i.e., 23% of other outputs scored the same or lower than it.
So far Altmetric has tracked 9,320 research outputs from this source. They receive a mean Attention Score of 2.7. This one has gotten more attention than average, scoring higher than 55% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 282,618 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 30th percentile – i.e., 30% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 7 others from the same source and published within six weeks on either side of this one.