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Mendeley readers
Chapter title |
Modification of Selenoprotein mRNAs by Cap Tri-methylation
|
---|---|
Chapter number | 9 |
Book title |
Selenoproteins
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Published in |
Methods in molecular biology, January 2018
|
DOI | 10.1007/978-1-4939-7258-6_9 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7257-9, 978-1-4939-7258-6
|
Authors |
Anne-Sophie Gribling-Burrer, Gilbert Eriani, Christine Allmang, Gribling-Burrer, Anne-Sophie, Eriani, Gilbert, Allmang, Christine |
Abstract |
Several selenoprotein mRNAs undergo 5' cap maturation events whereby their classical monomethylated m(7)G cap becomes trimethylated (m3(2,2,7)G) by the trimethylguanosine synthase 1 (Tgs1). Here, we describe immunoprecipitation methods for the detection of endogenous m3(2,2,7)G-capped selenoprotein mRNAs from total cell extracts or after polysome fractionation of cytoplasmic extracts. We have also developed a method for the in vitro cap hypermethylation of selenoprotein mRNA transcripts using purified Tgs1 enzyme. |
Mendeley readers
The data shown below were compiled from readership statistics for 13 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 13 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 3 | 23% |
Student > Ph. D. Student | 3 | 23% |
Unspecified | 1 | 8% |
Student > Bachelor | 1 | 8% |
Professor > Associate Professor | 1 | 8% |
Other | 0 | 0% |
Unknown | 4 | 31% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 3 | 23% |
Agricultural and Biological Sciences | 2 | 15% |
Environmental Science | 1 | 8% |
Unspecified | 1 | 8% |
Nursing and Health Professions | 1 | 8% |
Other | 1 | 8% |
Unknown | 4 | 31% |