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Mendeley readers
| Chapter title |
Overexpression of Recombinant Selenoproteins in E. coli
|
|---|---|
| Chapter number | 17 |
| Book title |
Selenoproteins
|
| Published in |
Methods in molecular biology, January 2018
|
| DOI | 10.1007/978-1-4939-7258-6_17 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-7257-9, 978-1-4939-7258-6
|
| Authors |
Qing Cheng, Elias S. J. Arnér, Cheng, Qing, Arnér, Elias S. J. |
| Abstract |
Expression of selenoproteins necessitates a process of decoding of a UGA codon from termination of translation to insertion of selenocysteine. The mechanisms of this process pose major challenges with regards to recombinant selenoprotein production in E. coli, which however can be overcome especially if the Sec residue is located close to the C-terminal end, as is the case for several naturally found selenoproteins. This chapter summarizes a method to achieve such a production. |
Mendeley readers
The data shown below were compiled from readership statistics for 15 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 15 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 4 | 27% |
| Student > Doctoral Student | 2 | 13% |
| Professor | 2 | 13% |
| Student > Ph. D. Student | 2 | 13% |
| Student > Master | 1 | 7% |
| Other | 1 | 7% |
| Unknown | 3 | 20% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 8 | 53% |
| Agricultural and Biological Sciences | 2 | 13% |
| Immunology and Microbiology | 2 | 13% |
| Unknown | 3 | 20% |