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Selenoproteins

Overview of attention for book
Cover of 'Selenoproteins'

Table of Contents

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    Book Overview
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    Chapter 1 SECISearch3 and Seblastian: In-Silico Tools to Predict SECIS Elements and Selenoproteins
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    Chapter 2 Selenoprofiles: A Computational Pipeline for Annotation of Selenoproteins
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    Chapter 3 SelGenAmic: An Algorithm for Selenoprotein Gene Assembly
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    Chapter 4 Selenocysteine tRNA[Ser]Sec, the Central Component of Selenoprotein Biosynthesis: Isolation, Identification, Modification, and Sequencing
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    Chapter 5 Identification and Characterization of Proteins that Bind to Selenoprotein 3′ UTRs
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    Chapter 6 Specific Chemical Approaches for Studying Mammalian Ribosomes Complexed with Ligands Involved in Selenoprotein Synthesis
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    Chapter 7 In Vitro Translation Assays for Selenocysteine Insertion
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    Chapter 8 Studying Selenoprotein mRNA Translation Using RNA-Seq and Ribosome Profiling
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    Chapter 9 Modification of Selenoprotein mRNAs by Cap Tri-methylation
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    Chapter 10 Total Selenium Quantification in Biological Samples by Inductively Coupled Plasma Mass Spectrometry (ICP-MS)
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    Chapter 11 Quantification of SeMet and SeCys in Biological Fluids and Tissues by Liquid Chromatography Coupled to Inductively Coupled Plasma Mass Spectrometry (HPLC-ICP MS)
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    Chapter 12 Simultaneous Speciation of Selenoproteins and Selenometabolites in Plasma and Serum
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    Chapter 13 Radioactive 75 Se Labeling and Detection of Selenoproteins
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    Chapter 14 Nonradioactive Isotopic Labeling and Tracing of Selenoproteins in Cultured Cell Lines
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    Chapter 15 Detection of Selenoproteins by Laser Ablation Inductively Coupled Plasma Mass Spectrometry (LA-ICP MS) in Immobilized pH Gradient (IPG) Strips
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    Chapter 16 Imaging of Selenium by Laser Ablation Inductively Coupled Plasma Mass Spectrometry (LA-ICP-MS) in 2-D Electrophoresis Gels and Biological Tissues
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    Chapter 17 Overexpression of Recombinant Selenoproteins in E. coli
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    Chapter 18 Preparation of Selenocysteine-Containing Forms of Human SELENOK and SELENOS
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    Chapter 19 Selenocysteine-Mediated Expressed Protein Ligation of SELENOM
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    Chapter 20 Monitoring of Methionine Sulfoxide Content and Methionine Sulfoxide Reductase Activity
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    Chapter 21 Selective Evaluation of Thioredoxin Reductase Enzymatic Activities
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    Chapter 22 Association of Single Nucleotide Polymorphisms in Selenoprotein Genes with Cancer Risk
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    Chapter 23 Identification of Genetic Disorders Causing Disruption of Selenoprotein Biosynthesis
Attention for Chapter 13: Radioactive 75 Se Labeling and Detection of Selenoproteins
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Chapter title
Radioactive 75 Se Labeling and Detection of Selenoproteins
Chapter number 13
Book title
Selenoproteins
Published in
Methods in molecular biology, January 2018
DOI 10.1007/978-1-4939-7258-6_13
Pubmed ID
Book ISBNs
978-1-4939-7257-9, 978-1-4939-7258-6
Authors

Sun Hee Yim, Ryuta Tobe, Anton A. Turanov, Bradley A. Carlson, Yim, Sun Hee, Tobe, Ryuta, Turanov, Anton A., Carlson, Bradley A.

Abstract

The trace element selenium (Se) is incorporated into proteins as the amino acid selenocysteine (Sec), which is cotranslationally inserted into specific proteins in response to a UGA codon. Proteins containing Sec at these specific positions are called selenoproteins. Most selenoproteins function as oxidoreductases, while some serve other important functions. There are 25 known selenoprotein genes in humans and 24 in mice. The use of Sec allows selenoproteins to be detected by a convenient method involving metabolic labeling with (75)Se. Labeling of cells and whole animals are used for the examination of selenoprotein expression profiles and the investigation of selenoprotein functions. In mammals, nonspecific (75)Se insertion is very low, and sensitivity and specificity of selenoprotein detection approaches that of Western blotting. This method allows for the examination of selenoprotein expression and Se metabolism in model and non-model organisms. Herein, we describe experimental protocols for analyzing selenoproteins by metabolic labeling with (75)Se both in vitro and in vivo. As an example, the procedure for metabolic labeling of HEK293T human embryonic kidney cells is described in detail. This approach remains a method of choice for the detection of selenoproteins in diverse settings.

Twitter Demographics

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Mendeley readers

The data shown below were compiled from readership statistics for 4 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 4 100%

Demographic breakdown

Readers by professional status Count As %
Student > Doctoral Student 2 50%
Researcher 1 25%
Professor > Associate Professor 1 25%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 3 75%
Veterinary Science and Veterinary Medicine 1 25%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 22 May 2018.
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#11,534,621
of 12,980,529 outputs
Outputs from Methods in molecular biology
#6,002
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Outputs of similar age
#233,940
of 268,365 outputs
Outputs of similar age from Methods in molecular biology
#5
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