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Notch Signaling

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Cover of 'Notch Signaling'

Table of Contents

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    Book Overview
  2. Altmetric Badge
    Chapter 1 Introduction to notch signaling.
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    Chapter 2 Genetic screens to identify new notch pathway mutants in Drosophila.
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    Chapter 3 Structure-function analysis of Drosophila notch using genomic rescue transgenes.
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    Chapter 4 Overview of Genetic Tools and Techniques to Study Notch Signaling in Mice
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    Chapter 5 Immunohistochemical Tools and Techniques to Visualize Notch in Drosophila melanogaster.
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    Chapter 6 Antibody uptake assay and in vivo imaging to study intracellular trafficking of notch and delta in Drosophila.
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    Chapter 7 Tracking Trafficking of Notch and Its Ligands in Mammalian Cells
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    Chapter 8 Visualizing notch signaling in vivo in Drosophila tissues.
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    Chapter 9 Monitoring Notch Activity in the Mouse
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    Chapter 10 Notch signaling assays in Drosophila cultured cell lines.
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    Chapter 11 Monitoring Notch Activation in Cultured Mammalian Cells: Transcriptional Reporter Assays
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    Chapter 12 Monitoring notch activation in cultured Mammalian cells: luciferase complementation imaging assays.
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    Chapter 13 Visualization of Notch Signaling Oscillation in Cells and Tissues
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    Chapter 14 Proteomic analysis of the notch interactome.
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    Chapter 15 Bacterial Expression and In Vitro Refolding of Limited Fragments of the Notch Receptor and Its Ligands
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    Chapter 16 Analyzing the Posttranslational Modification Status of Notch Using Mass Spectrometry
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    Chapter 17 Assay to Probe Proteolytic Processing of Notch by γ-Secretase
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    Chapter 18 Analyzing the nuclear complexes of notch signaling by electrophoretic mobility shift assay.
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    Chapter 19 Identifying Direct Notch Transcriptional Targets Using the GSI-Washout Assay
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    Chapter 20 Probing the epigenetic status at notch target genes.
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    Chapter 21 Notch-ligand binding assays in Drosophila cells.
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    Chapter 22 Modeling notch signaling: a practical tutorial.
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    Chapter 23 Small Molecules That Inhibit Notch Signaling
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    Chapter 24 Application and Evaluation of Anti-Notch Antibodies to Modulate Notch Signaling
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    Chapter 25 Application of Anti-Ligand Antibodies to Inhibit Notch Signaling
Attention for Chapter 2: Genetic screens to identify new notch pathway mutants in Drosophila.
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Chapter title
Genetic screens to identify new notch pathway mutants in Drosophila.
Chapter number 2
Book title
Notch Signaling
Published in
Methods in molecular biology, January 2014
DOI 10.1007/978-1-4939-1139-4_2
Pubmed ID
Book ISBNs
978-1-4939-1138-7, 978-1-4939-1139-4
Authors

Nikolaos Giagtzoglou, Giagtzoglou, Nikolaos

Abstract

Notch signaling controls a wide range of developmental processes, including proliferation, apoptosis, and cell fate specification during both development and adult tissue homeostasis. The functional versatility of the Notch signaling pathway is tightly linked with the complexity of its regulation in different cellular contexts. To unravel the complexity of Notch signaling, it is important to identify the different components of the Notch signaling pathway. A powerful strategy to accomplish this task is based on genetic screens. Given that the developmental context of signaling is important, these screens should be customized to specific cell populations or tissues. Here, I describe how to perform F1 clonal forward genetic screens in Drosophila to identify novel components of the Notch signaling pathway. These screens combine a classical EMS (ethyl methanesulfonate) chemical mutagenesis protocol along with clonal analysis via FRT-mediated mitotic recombination. These F1 clonal screens allow rapid phenotypic screening within clones of mutant cells induced at specific developmental stages and in tissues of interest, bypassing the pleiotropic effects of isolated mutations. More importantly, since EMS mutations have been notoriously difficult to map to specific genes in the past, I briefly discuss mapping methods that allow rapid identification of the causative mutations.

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X Demographics

The data shown below were collected from the profiles of 2 X users who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 8 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 8 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 2 25%
Researcher 2 25%
Student > Bachelor 1 13%
Professor > Associate Professor 1 13%
Unknown 2 25%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 4 50%
Agricultural and Biological Sciences 2 25%
Unknown 2 25%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 10 March 2015.
All research outputs
#15,866,607
of 23,577,654 outputs
Outputs from Methods in molecular biology
#5,555
of 13,410 outputs
Outputs of similar age
#193,937
of 309,121 outputs
Outputs of similar age from Methods in molecular biology
#210
of 576 outputs
Altmetric has tracked 23,577,654 research outputs across all sources so far. This one is in the 22nd percentile – i.e., 22% of other outputs scored the same or lower than it.
So far Altmetric has tracked 13,410 research outputs from this source. They receive a mean Attention Score of 3.4. This one is in the 43rd percentile – i.e., 43% of its peers scored the same or lower than it.
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We're also able to compare this research output to 576 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 51% of its contemporaries.