| Chapter title |
An In Vitro Assay for Outer Membrane Protein Assembly by the BAM Complex
|
|---|---|
| Chapter number | 16 |
| Book title |
The BAM Complex
|
| Published in |
Methods in molecular biology, January 2015
|
| DOI | 10.1007/978-1-4939-2871-2_16 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-2870-5, 978-1-4939-2871-2
|
| Authors |
Giselle Roman-Hernandez, Harris D. Bernstein, Roman-Hernandez, Giselle, Bernstein, Harris D. |
| Abstract |
To elucidate the mechanism of a biochemical process it is often essential to reconstitute the reaction in vitro using the minimal set of factors required to drive the reaction to completion. Here, we describe a method to reconstitute the folding and membrane integration of bacterial outer membrane (OM) proteins that have a characteristic β-barrel structure. In this method the BAM complex, a heteroligomer that catalyzes the membrane integration of β-barrel proteins, is first purified and inserted into small lipid vesicles. Denatured OM proteins are then assembled and integrated into the vesicles in the presence of a molecular chaperone called SurA. |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 18 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 8 | 44% |
| Researcher | 2 | 11% |
| Student > Bachelor | 2 | 11% |
| Unspecified | 1 | 6% |
| Student > Master | 1 | 6% |
| Other | 1 | 6% |
| Unknown | 3 | 17% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 10 | 56% |
| Agricultural and Biological Sciences | 3 | 17% |
| Earth and Planetary Sciences | 1 | 6% |
| Chemistry | 1 | 6% |
| Unknown | 3 | 17% |