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The BAM Complex

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Cover of 'The BAM Complex'

Table of Contents

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    Book Overview
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    Chapter 1 The β-Barrel Assembly Machinery Complex
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    Chapter 2 Yeast Mitochondria as a Model System to Study the Biogenesis of Bacterial β-Barrel Proteins.
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    Chapter 3 Experimental Methods for Studying the BAM Complex in Neisseria meningitidis
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    Chapter 4 Heat Modifiability of Outer Membrane Proteins from Gram-Negative Bacteria
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    Chapter 5 The Role of a Destabilized Membrane for OMP Insertion
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    Chapter 6 Treponema pallidum in Gel Microdroplets: A Method for Topological Analysis of BamA (TP0326) and Localization of Rare Outer Membrane Proteins
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    Chapter 7 Analyzing the Role of Periplasmic Folding Factors in the Biogenesis of OMPs and Members of the Type V Secretion System
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    Chapter 8 An In Vitro Assay for Substrate Translocation by FhaC in Liposomes
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    Chapter 9 Measuring Cell–Cell Binding Using Flow-Cytometry
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    Chapter 10 Methods to Characterize Folding and Function of BamA Cross-Link Mutants
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    Chapter 11 The BAM Complex
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    Chapter 12 Assessing the Outer Membrane Insertion and Folding of Multimeric Transmembrane β-Barrel Proteins
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    Chapter 13 The Expression, Purification, and Structure Determination of BamA from E. coli
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    Chapter 14 Expression and Purification of the Individual Bam Components BamB–E
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    Chapter 15 Structure Determination of the BAM Complex Accessory Lipoproteins BamB–E
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    Chapter 16 An In Vitro Assay for Outer Membrane Protein Assembly by the BAM Complex
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    Chapter 17 Identification of BamC on the Surface of E. coli
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    Chapter 18 The BAM Complex
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    Chapter 19 Expression, Purification, and Screening of BamE, a Component of the BAM Complex, for Structural Characterization
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    Chapter 20 Purification and Bicelle Crystallization for Structure Determination of the E. coli Outer Membrane Protein TamA
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    Chapter 21 Strategies for the Analysis of Bam Recognition Motifs in Outer Membrane Proteins
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    Chapter 22 Summary and Future Directions
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    Chapter 23 Erratum to: The Role of a Destabilized Membrane for OMP Insertion
Attention for Chapter 7: Analyzing the Role of Periplasmic Folding Factors in the Biogenesis of OMPs and Members of the Type V Secretion System
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Chapter title
Analyzing the Role of Periplasmic Folding Factors in the Biogenesis of OMPs and Members of the Type V Secretion System
Chapter number 7
Book title
The BAM Complex
Published in
Methods in molecular biology, January 2015
DOI 10.1007/978-1-4939-2871-2_7
Pubmed ID
Book ISBNs
978-1-4939-2870-5, 978-1-4939-2871-2
Authors

Gustavo Bodelón, Elvira Marín, Luis Ángel Fernández, Bodelón, Gustavo, Marín, Elvira, Fernández, Luis Ángel

Abstract

The outer membrane (OM) of gram-negative bacteria is highly packed with OM proteins (OMPs) and the trafficking and assembly of OMPs in gram-negative bacteria is a subject of intense research. Structurally, OMPs vary in the number of β-strands and in the size and complexity of extra-membrane domains, with extreme examples being the members of the type V protein secretion system (T5SS), such as the autotransporter (AT) and intimin/invasin families of secreted proteins, in which a large extracellular "passenger" domain is linked to a β-barrel that inserts in the OM. Despite their structural and functional diversity, OMPs interact in the periplasm with a relatively small set of protein chaperones that facilitate their transport from the inner membrane (IM) to the β-barrel assembly machinery (BAM complex), preventing aggregation and assisting their folding in various aspects including disulfide bond formation. This chapter is focused on the periplasmic folding factors involved in the biogenesis of integral OMPs and members of T5SS in E. coli, which are used as a model system in this field. Background information on these periplasmic folding factors is provided along with genetic methods to generate conditional mutants that deplete these factors from E. coli and biochemical methods to analyze the folding, surface display, disulfide formation and oligomerization state of OMPs/T5SS in these mutants.

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Mendeley readers

The data shown below were compiled from readership statistics for 8 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 8 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 2 25%
Unspecified 1 13%
Other 1 13%
Professor 1 13%
Lecturer 1 13%
Other 2 25%
Readers by discipline Count As %
Unspecified 1 13%
Biochemistry, Genetics and Molecular Biology 1 13%
Business, Management and Accounting 1 13%
Agricultural and Biological Sciences 1 13%
Immunology and Microbiology 1 13%
Other 2 25%
Unknown 1 13%