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Single Cell Protein Analysis

Overview of attention for book
Cover of 'Single Cell Protein Analysis'

Table of Contents

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    Book Overview
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    Chapter 1 Single-Cell Western Blotting
  3. Altmetric Badge
    Chapter 2 A Microfluidic Device for Immunoassay-Based Protein Analysis of Single E. coli Bacteria
  4. Altmetric Badge
    Chapter 3 Enzyme-Linked ImmunoSpot (ELISpot) for Single-Cell Analysis
  5. Altmetric Badge
    Chapter 4 Single Cell Protein Analysis
  6. Altmetric Badge
    Chapter 5 Single Cell Protein Analysis
  7. Altmetric Badge
    Chapter 6 Microfluidic Flow Cytometry for Single-Cell Protein Analysis
  8. Altmetric Badge
    Chapter 7 Microfluidic Image Cytometry for Single-Cell Phenotyping of Human Pluripotent Stem Cells
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    Chapter 8 Characterizing Phenotypes and Signaling Networks of Single Human Cells by Mass Cytometry.
  10. Altmetric Badge
    Chapter 9 Multiplexed Peptide-MHC Tetramer Staining with Mass Cytometry.
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    Chapter 10 Imaging and Mapping of Tissue Constituents at the Single-Cell Level Using MALDI MSI and Quantitative Laser Scanning Cytometry
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    Chapter 11 SPLIFF: A Single-Cell Method to Map Protein-Protein Interactions in Time and Space.
  13. Altmetric Badge
    Chapter 12 Microfluidic Proximity Ligation Assay for Profiling Signaling Networks with Single-Cell Resolution
  14. Altmetric Badge
    Chapter 13 Dynamics and Interactions of Individual Proteins in the Membrane of Single, Living Cells
  15. Altmetric Badge
    Chapter 14 Microfluidics-Enabled Enzyme Activity Measurement in Single Cells
  16. Altmetric Badge
    Chapter 15 Microfluidic Chemical Cytometry for Enzyme Assays of Single Cells
  17. Altmetric Badge
    Chapter 16 Quantitative Detection of Nucleocytoplasmic Transport of Native Proteins in Single Cells
Attention for Chapter 4: Single Cell Protein Analysis
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Chapter title
Single Cell Protein Analysis
Chapter number 4
Book title
Single Cell Protein Analysis
Published in
Methods in molecular biology, January 2015
DOI 10.1007/978-1-4939-2987-0_4
Pubmed ID
Book ISBNs
978-1-4939-2986-3, 978-1-4939-2987-0
Authors

Ullal, Adeeti V, Weissleder, Ralph, Adeeti V. Ullal, Ralph Weissleder

Abstract

We describe a DNA-barcoded antibody sensing technique for single cell protein analysis in which the barcodes are photocleaved and digitally detected without amplification steps (Ullal et al., Sci Transl Med 6:219, 2014). After photocleaving the unique ~70mer DNA barcodes we use a fluorescent hybridization technology for detection, similar to what is commonly done for nucleic acid readouts. This protocol offers a simple method for multiplexed protein detection using 100+ antibodies and can be performed on clinical samples as well as single cells.

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X Demographics

The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 12 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 12 100%

Demographic breakdown

Readers by professional status Count As %
Lecturer > Senior Lecturer 2 17%
Researcher 2 17%
Student > Ph. D. Student 1 8%
Lecturer 1 8%
Student > Master 1 8%
Other 1 8%
Unknown 4 33%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 5 42%
Chemistry 1 8%
Neuroscience 1 8%
Medicine and Dentistry 1 8%
Unknown 4 33%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 19 August 2016.
All research outputs
#18,467,727
of 22,883,326 outputs
Outputs from Methods in molecular biology
#7,923
of 13,131 outputs
Outputs of similar age
#256,121
of 353,371 outputs
Outputs of similar age from Methods in molecular biology
#481
of 997 outputs
Altmetric has tracked 22,883,326 research outputs across all sources so far. This one is in the 11th percentile – i.e., 11% of other outputs scored the same or lower than it.
So far Altmetric has tracked 13,131 research outputs from this source. They receive a mean Attention Score of 3.4. This one is in the 24th percentile – i.e., 24% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 353,371 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 16th percentile – i.e., 16% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 997 others from the same source and published within six weeks on either side of this one. This one is in the 36th percentile – i.e., 36% of its contemporaries scored the same or lower than it.