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Single Cell Protein Analysis

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Cover of 'Single Cell Protein Analysis'

Table of Contents

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    Book Overview
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    Chapter 1 Single-Cell Western Blotting
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    Chapter 2 A Microfluidic Device for Immunoassay-Based Protein Analysis of Single E. coli Bacteria
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    Chapter 3 Enzyme-Linked ImmunoSpot (ELISpot) for Single-Cell Analysis
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    Chapter 4 Single Cell Protein Analysis
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    Chapter 5 Single Cell Protein Analysis
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    Chapter 6 Microfluidic Flow Cytometry for Single-Cell Protein Analysis
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    Chapter 7 Microfluidic Image Cytometry for Single-Cell Phenotyping of Human Pluripotent Stem Cells
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    Chapter 8 Characterizing Phenotypes and Signaling Networks of Single Human Cells by Mass Cytometry.
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    Chapter 9 Multiplexed Peptide-MHC Tetramer Staining with Mass Cytometry.
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    Chapter 10 Imaging and Mapping of Tissue Constituents at the Single-Cell Level Using MALDI MSI and Quantitative Laser Scanning Cytometry
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    Chapter 11 SPLIFF: A Single-Cell Method to Map Protein-Protein Interactions in Time and Space.
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    Chapter 12 Microfluidic Proximity Ligation Assay for Profiling Signaling Networks with Single-Cell Resolution
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    Chapter 13 Dynamics and Interactions of Individual Proteins in the Membrane of Single, Living Cells
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    Chapter 14 Microfluidics-Enabled Enzyme Activity Measurement in Single Cells
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    Chapter 15 Microfluidic Chemical Cytometry for Enzyme Assays of Single Cells
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    Chapter 16 Quantitative Detection of Nucleocytoplasmic Transport of Native Proteins in Single Cells
Attention for Chapter 7: Microfluidic Image Cytometry for Single-Cell Phenotyping of Human Pluripotent Stem Cells
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Chapter title
Microfluidic Image Cytometry for Single-Cell Phenotyping of Human Pluripotent Stem Cells
Chapter number 7
Book title
Single Cell Protein Analysis
Published in
Methods in molecular biology, January 2015
DOI 10.1007/978-1-4939-2987-0_7
Pubmed ID
Book ISBNs
978-1-4939-2986-3, 978-1-4939-2987-0
Authors

Yasumasa Mashimo, Ken-ichiro Kamei

Abstract

A microfluidic human pluripotent stem cell (hPSC) array has been developed for robust and reproducible hPSC culture methods to assess chemically defined serum- and feeder-free culture conditions. This microfluidic platform, combined with image cytometry, enables the systematic analysis of multiple simultaneously detected marker expression in individual cells, for screening of various chemically defined media across hPSC lines, and the study of phenotypic responses.

Mendeley readers

The data shown below were compiled from readership statistics for 9 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 9 100%

Demographic breakdown

Readers by professional status Count As %
Other 3 33%
Student > Bachelor 2 22%
Researcher 2 22%
Professor > Associate Professor 2 22%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 4 44%
Engineering 2 22%
Agricultural and Biological Sciences 1 11%
Immunology and Microbiology 1 11%
Pharmacology, Toxicology and Pharmaceutical Science 1 11%
Other 0 0%