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Mendeley readers
| Chapter title |
Microfluidic Chemical Cytometry for Enzyme Assays of Single Cells
|
|---|---|
| Chapter number | 15 |
| Book title |
Single Cell Protein Analysis
|
| Published in |
Methods in molecular biology, January 2015
|
| DOI | 10.1007/978-1-4939-2987-0_15 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-2986-3, 978-1-4939-2987-0
|
| Authors |
Livia Shehaj, Lorena Lazo de la Vega, Michelle L. Kovarik |
| Abstract |
Cellular heterogeneity occurs, and should be probed, at multiple levels of cellular structure and physiology from the genome to enzyme activity. In particular, single-cell measures of protein levels are complemented by single-cell measurements of the activity of these proteins. Microfluidic assays of enzyme activity at the single-cell level combine moderate to high throughput with low dead volumes and the potential for automation. Herein, we describe the steps required to fabricate and operate a microfluidic device for chemical cytometry of fluorescent or fluorogenic reporters of enzyme activity in individual cells. |
Mendeley readers
The data shown below were compiled from readership statistics for 5 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 5 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 2 | 40% |
| Researcher | 2 | 40% |
| Student > Master | 1 | 20% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 2 | 40% |
| Chemistry | 1 | 20% |
| Medicine and Dentistry | 1 | 20% |
| Engineering | 1 | 20% |