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Mendeley readers
Chapter title |
A Microfluidic Device for Immunoassay-Based Protein Analysis of Single E. coli Bacteria
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Chapter number | 2 |
Book title |
Single Cell Protein Analysis
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Published in |
Methods in molecular biology, January 2015
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DOI | 10.1007/978-1-4939-2987-0_2 |
Pubmed ID | |
Book ISBNs |
978-1-4939-2986-3, 978-1-4939-2987-0
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Authors |
Simone Stratz, Petra S. Dittrich |
Abstract |
We present a method suitable for quantitative analysis of intracellular proteins, metabolites and secondary messengers of single bacterial cells. The method integrates the concept of immunoassays on a microfluidic device that facilitates single cell trapping and isolating in a small volume of a few tens of picoliters. Combination of the benefits of microfluidic systems for single cell analysis with the high analytical selectivity and sensitivity of immunoassays enables the detection of even low abundant intracellular analytes which occur only at a few hundred copies per bacterium. |
Mendeley readers
The data shown below were compiled from readership statistics for 7 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 7 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 3 | 43% |
Student > Master | 2 | 29% |
Researcher | 1 | 14% |
Unknown | 1 | 14% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 2 | 29% |
Engineering | 2 | 29% |
Chemistry | 1 | 14% |
Agricultural and Biological Sciences | 1 | 14% |
Unknown | 1 | 14% |