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Single Cell Protein Analysis

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Cover of 'Single Cell Protein Analysis'

Table of Contents

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    Book Overview
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    Chapter 1 Single-Cell Western Blotting
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    Chapter 2 A Microfluidic Device for Immunoassay-Based Protein Analysis of Single E. coli Bacteria
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    Chapter 3 Enzyme-Linked ImmunoSpot (ELISpot) for Single-Cell Analysis
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    Chapter 4 Single Cell Protein Analysis
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    Chapter 5 Single Cell Protein Analysis
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    Chapter 6 Microfluidic Flow Cytometry for Single-Cell Protein Analysis
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    Chapter 7 Microfluidic Image Cytometry for Single-Cell Phenotyping of Human Pluripotent Stem Cells
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    Chapter 8 Characterizing Phenotypes and Signaling Networks of Single Human Cells by Mass Cytometry.
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    Chapter 9 Multiplexed Peptide-MHC Tetramer Staining with Mass Cytometry.
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    Chapter 10 Imaging and Mapping of Tissue Constituents at the Single-Cell Level Using MALDI MSI and Quantitative Laser Scanning Cytometry
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    Chapter 11 SPLIFF: A Single-Cell Method to Map Protein-Protein Interactions in Time and Space.
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    Chapter 12 Microfluidic Proximity Ligation Assay for Profiling Signaling Networks with Single-Cell Resolution
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    Chapter 13 Dynamics and Interactions of Individual Proteins in the Membrane of Single, Living Cells
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    Chapter 14 Microfluidics-Enabled Enzyme Activity Measurement in Single Cells
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    Chapter 15 Microfluidic Chemical Cytometry for Enzyme Assays of Single Cells
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    Chapter 16 Quantitative Detection of Nucleocytoplasmic Transport of Native Proteins in Single Cells
Attention for Chapter 13: Dynamics and Interactions of Individual Proteins in the Membrane of Single, Living Cells
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Chapter title
Dynamics and Interactions of Individual Proteins in the Membrane of Single, Living Cells
Chapter number 13
Book title
Single Cell Protein Analysis
Published in
Methods in molecular biology, January 2015
DOI 10.1007/978-1-4939-2987-0_13
Pubmed ID
Book ISBNs
978-1-4939-2986-3, 978-1-4939-2987-0
Authors

Stephen Anthony, Amanda Carroll-Portillo, Jerilyn Timlin

Abstract

Total internal reflection fluorescence (TIRF) microscopy is a powerful technique for interrogating protein dynamics in the membranes of living single cells. Receptor-ligand interactions are of particular interest for improving our understanding of cell signaling networks in a variety of applications. Here, we describe methods for fluorescently labeling individual receptors and their ligands, conducting single-molecule TIRF microscopy of receptors and ligands in single, living cells, and importantly, performing image analysis on the resulting time sequence of images to extract quantitative dynamics. While we use Toll-like receptor 4 and its ligand lipopolysaccharide as a specific example, the methods are general and readily extendable to other receptor-ligand systems of importance in cellular biology.

Mendeley readers

The data shown below were compiled from readership statistics for 36 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United States 1 3%
Unknown 35 97%

Demographic breakdown

Readers by professional status Count As %
Researcher 12 33%
Student > Ph. D. Student 11 31%
Student > Master 4 11%
Student > Postgraduate 2 6%
Professor 1 3%
Other 3 8%
Unknown 3 8%
Readers by discipline Count As %
Agricultural and Biological Sciences 7 19%
Biochemistry, Genetics and Molecular Biology 7 19%
Immunology and Microbiology 5 14%
Engineering 3 8%
Pharmacology, Toxicology and Pharmaceutical Science 2 6%
Other 5 14%
Unknown 7 19%