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Synthetic mRNA

Overview of attention for book
Attention for Chapter 4: Preparation of Functional, Fluorescently Labeled mRNA Capped with Anthraniloyl-m 7 GpppG
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About this Attention Score

  • Above-average Attention Score compared to outputs of the same age (55th percentile)
  • Good Attention Score compared to outputs of the same age and source (79th percentile)

Mentioned by

patent
1 patent

Citations

dimensions_citation
1 Dimensions

Readers on

mendeley
10 Mendeley
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Chapter title
Preparation of Functional, Fluorescently Labeled mRNA Capped with Anthraniloyl-m 7 GpppG
Chapter number 4
Book title
Synthetic mRNA
Published in
Methods in molecular biology, January 2016
DOI 10.1007/978-1-4939-3625-0_4
Pubmed ID
Book ISBNs
978-1-4939-3623-6, 978-1-4939-3625-0
Authors

Artem V. Domashevskiy, David J. Rodriguez, Dilantha Gunawardana, Dixie J. Goss, Domashevskiy, Artem V., Rodriguez, David J., Gunawardana, Dilantha, Goss, Dixie J.

Abstract

Fluorescent mRNA molecules offer a wide range of applications for studying capping/decapping reactions, translation, and other biophysical studies. Furthermore, fluorescent tags prove invaluable for tracking RNA molecules in cells. Here, we describe an efficient synthesis of a fluorescent cap analog, anthranioyl-GTP, its purification, and in vitro cap labeling of transcribed mRNA catalyzed by the recombinant vaccinia capping enzyme to produce anthranioyl-m(7)GpppG-capped RNA.

Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 10 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 10 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 4 40%
Researcher 2 20%
Student > Doctoral Student 1 10%
Professor 1 10%
Lecturer > Senior Lecturer 1 10%
Other 0 0%
Unknown 1 10%
Readers by discipline Count As %
Agricultural and Biological Sciences 4 40%
Chemistry 2 20%
Biochemistry, Genetics and Molecular Biology 1 10%
Unknown 3 30%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 3. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 18 January 2023.
All research outputs
#7,746,777
of 23,556,846 outputs
Outputs from Methods in molecular biology
#2,425
of 13,398 outputs
Outputs of similar age
#124,987
of 396,990 outputs
Outputs of similar age from Methods in molecular biology
#272
of 1,472 outputs
Altmetric has tracked 23,556,846 research outputs across all sources so far. This one is in the 44th percentile – i.e., 44% of other outputs scored the same or lower than it.
So far Altmetric has tracked 13,398 research outputs from this source. They receive a mean Attention Score of 3.4. This one has done well, scoring higher than 75% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 396,990 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 55% of its contemporaries.
We're also able to compare this research output to 1,472 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 79% of its contemporaries.