Chapter title |
Synthetic mRNA
|
---|---|
Chapter number | 15 |
Book title |
Synthetic mRNA
|
Published in |
Methods in molecular biology, January 2016
|
DOI | 10.1007/978-1-4939-3625-0_15 |
Pubmed ID | |
Book ISBNs |
978-1-4939-3623-6, 978-1-4939-3625-0
|
Authors |
Devi, Gayathri R, Nath, Sritama, Devi, Gayathri R., Gayathri R. Devi, Sritama Nath |
Abstract |
Dendritic cell (DC)-based vaccines are commonly used for cancer immunotherapy. To prepare vaccines, DCs are pulsed or transfected with either: (a) defined peptides of tumor-associated antigens, (b) total protein isolated from the tumor cell, (c) autologous total RNA isolated from the tumor cell, (d) synthetic tumor-antigen-encoding mRNA, or (e) genes that encode for specific tumor-associated antigens. Introduction of tumor-associated antigen(s) and subsequent generation of mature DCs that can stimulate tumor-antigen-specific cytotoxic T lymphocytes comprise the critical steps of cancer vaccine preparation. Here, we described a method of: (a) preparing and delivering synthetic FOXP3 mRNA into human DCs, (b) generating mature DCs, |
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