Chapter title |
Synthetic mRNA
|
---|---|
Chapter number | 7 |
Book title |
Synthetic mRNA
|
Published in |
Methods in molecular biology, January 2016
|
DOI | 10.1007/978-1-4939-3625-0_7 |
Pubmed ID | |
Book ISBNs |
978-1-4939-3623-6, 978-1-4939-3625-0
|
Authors |
Benteyn, Daphné, Heirman, Carlo, Thielemans, Kris, Bonehill, Aude, Daphné Benteyn, Carlo Heirman, Kris Thielemans, Aude Bonehill |
Abstract |
Dendritic cells (DCs) are the orchestrators of the immune system and are frequently used in clinical trials in order to boost the immune system in cancer patients. Among several available techniques for DC modification, mRNA electroporation is an interesting technique due to the favorable characteristics of mRNA. Antigen expression level and duration can be increased by multiple optimizations of an antigen-encoding mRNA template. Here, we describe different molecular modifications to a WT1-encoding mRNA construct in order to increase antigen expression and the subsequent introduction of mRNA into DCs. |
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