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Monoclonal Antibodies

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Cover of 'Monoclonal Antibodies'

Table of Contents

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    Book Overview
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    Chapter 1 Antigen Production for Monoclonal Antibody Generation
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    Chapter 2 Method for the Generation of Antibodies Specific for Site and Posttranslational Modifications
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    Chapter 3 Immunization, Hybridoma Generation, and Selection for Monoclonal Antibody Production
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    Chapter 4 Hybridoma Technology for the Generation of Rodent mAbs via Classical Fusion
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    Chapter 5 Generation of Rabbit Monoclonal Antibodies
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    Chapter 6 Screening Hybridomas for Cell Surface Antigens by High-Throughput Homogeneous Assay and Flow Cytometry
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    Chapter 7 Screening and Subcloning of High Producer Transfectomas Using Semisolid Media and Automated Colony Picker
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    Chapter 8 Design and Generation of Synthetic Antibody Libraries for Phage Display
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    Chapter 9 Selection and Screening Using Antibody Phage Display Libraries
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    Chapter 10 Yeast surface display for antibody isolation: library construction, library screening, and affinity maturation.
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    Chapter 11 Human B cell immortalization for monoclonal antibody production.
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    Chapter 12 Using Next-Generation Sequencing for Discovery of High-Frequency Monoclonal Antibodies in the Variable Gene Repertoires from Immunized Mice
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    Chapter 13 Cloning, Reformatting, and Small-Scale Expression of Monoclonal Antibody Isolated from Mouse, Rat, or Hamster Hybridoma
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    Chapter 14 Cloning of Recombinant Monoclonal Antibodies from Hybridomas in a Single Mammalian Expression Plasmid
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    Chapter 15 Monoclonal Antibody Purification by Ceramic Hydroxyapatite Chromatography
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    Chapter 16 Rapid Purification of Monoclonal Antibodies Using Magnetic Microspheres
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    Chapter 17 Generation of Cell Lines for Monoclonal Antibody Production
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    Chapter 18 Expression and Purification of Recombinant Antibody Formats and Antibody Fusion Proteins
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    Chapter 19 Purification of Antibodies and Antibody Fragments Using CaptureSelect ™ Affinity Resins
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    Chapter 20 Reformatting of scFv Antibodies into the scFv-Fc Format and Their Downstream Purification.
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    Chapter 21 Antibody V and C Domain Sequence, Structure, and Interaction Analysis with Special Reference to IMGT ®
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    Chapter 22 Measuring Antibody Affinities as Well as the Active Concentration of Antigens Present on a Cell Surface
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    Chapter 23 Determination of Antibody Structures
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    Chapter 24 Affinity Maturation of Monoclonal Antibodies by Multi-Site-Directed Mutagenesis
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    Chapter 25 Epitope mapping with membrane-bound synthetic overlapping peptides.
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    Chapter 26 Epitope Mapping by Epitope Excision, Hydrogen/Deuterium Exchange, and Peptide-Panning Techniques Combined with In Silico Analysis
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    Chapter 27 Fine Epitope Mapping Based on Phage Display and Extensive Mutagenesis of the Target Antigen
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    Chapter 28 Epitope Mapping with Random Phage Display Library
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    Chapter 29 Epitope Mapping of Monoclonal and Polyclonal Antibodies Using Bacterial Cell Surface Display
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    Chapter 30 Ion Exchange-High-Performance Liquid Chromatography (IEX-HPLC)
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    Chapter 31 Size Exclusion-High-Performance Liquid Chromatography (SEC-HPLC)
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    Chapter 32 N-Glycosylation Characterization by Liquid Chromatography with Mass Spectrometry
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    Chapter 33 Fc Engineering of Antibodies and Antibody Derivatives by Primary Sequence Alteration and Their Functional Characterization
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    Chapter 34 Labeling and Use of Monoclonal Antibodies in Immunofluorescence: Protocols for Cytoskeletal and Nuclear Antigens
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    Chapter 35 Generation and Use of Antibody Fragments for Structural Studies of Proteins Refractory to Crystallization
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    Chapter 36 Antibody array generation and use.
Attention for Chapter 36: Antibody array generation and use.
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Chapter title
Antibody array generation and use.
Chapter number 36
Book title
Monoclonal Antibodies
Published in
Methods in molecular biology, February 2014
DOI 10.1007/978-1-62703-992-5_36
Pubmed ID
Book ISBNs
978-1-62703-991-8, 978-1-62703-992-5
Authors

Borrebaeck CA, Wingren C, Carl A. K. Borrebaeck, Christer Wingren

Abstract

Affinity proteomics, represented by antibody arrays, is a multiplex technology for high-throughput protein expression profiling of crude proteomes in a highly specific, sensitive, and miniaturized manner. The antibodies are individually deposited in an ordered pattern, an array, onto a solid support. Next, the sample is added, and any specifically bound proteins are detected and quantified using mainly fluorescence as the mode of detection. The binding pattern is then converted into a relative protein expression map, or protein atlas, delineating the composition of the sample at the molecular level. The technology provides unique opportunities for various applications, such as protein expression profiling, biomarker discovery, disease diagnostics, prognostics, evidence-based therapy selection, and disease monitoring. Here, we describe the generation and use of planar antibody arrays for serum protein profiling.

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Geographical breakdown

Country Count As %
South Africa 1 33%
Unknown 2 67%

Demographic breakdown

Readers by professional status Count As %
Student > Master 1 33%
Student > Ph. D. Student 1 33%
Unspecified 1 33%
Readers by discipline Count As %
Unspecified 1 33%
Agricultural and Biological Sciences 1 33%
Medicine and Dentistry 1 33%

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