Monoclonal Antibodies

Table of Contents

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   Book Overview
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   Chapter 1 Antigen Production for Monoclonal Antibody Generation
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   Chapter 2 Method for the Generation of Antibodies Specific for Site and Posttranslational Modifications
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   Chapter 3 Immunization, Hybridoma Generation, and Selection for Monoclonal Antibody Production
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   Chapter 4 Hybridoma Technology for the Generation of Rodent mAbs via Classical Fusion
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   Chapter 5 Generation of Rabbit Monoclonal Antibodies
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   Chapter 6 Screening Hybridomas for Cell Surface Antigens by High-Throughput Homogeneous Assay and Flow Cytometry
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   Chapter 7 Screening and Subcloning of High Producer Transfectomas Using Semisolid Media and Automated Colony Picker
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   Chapter 8 Design and Generation of Synthetic Antibody Libraries for Phage Display
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    Chapter 9 Selection and Screening Using Antibody Phage Display Libraries
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    Chapter 10 Yeast surface display for antibody isolation: library construction, library screening, and affinity maturation.
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    Chapter 11 Human B cell immortalization for monoclonal antibody production.
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    Chapter 12 Using Next-Generation Sequencing for Discovery of High-Frequency Monoclonal Antibodies in the Variable Gene Repertoires from Immunized Mice
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    Chapter 13 Cloning, Reformatting, and Small-Scale Expression of Monoclonal Antibody Isolated from Mouse, Rat, or Hamster Hybridoma
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    Chapter 14 Cloning of Recombinant Monoclonal Antibodies from Hybridomas in a Single Mammalian Expression Plasmid
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    Chapter 15 Monoclonal Antibody Purification by Ceramic Hydroxyapatite Chromatography
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    Chapter 16 Rapid Purification of Monoclonal Antibodies Using Magnetic Microspheres
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    Chapter 17 Generation of Cell Lines for Monoclonal Antibody Production
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    Chapter 18 Expression and Purification of Recombinant Antibody Formats and Antibody Fusion Proteins
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    Chapter 19 Purification of Antibodies and Antibody Fragments Using CaptureSelect ™ Affinity Resins
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    Chapter 20 Reformatting of scFv Antibodies into the scFv-Fc Format and Their Downstream Purification.
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    Chapter 21 Antibody V and C Domain Sequence, Structure, and Interaction Analysis with Special Reference to IMGT ®
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    Chapter 22 Measuring Antibody Affinities as Well as the Active Concentration of Antigens Present on a Cell Surface
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    Chapter 23 Determination of Antibody Structures
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    Chapter 24 Affinity Maturation of Monoclonal Antibodies by Multi-Site-Directed Mutagenesis
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    Chapter 25 Epitope mapping with membrane-bound synthetic overlapping peptides.
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    Chapter 26 Epitope Mapping by Epitope Excision, Hydrogen/Deuterium Exchange, and Peptide-Panning Techniques Combined with In Silico Analysis
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    Chapter 27 Fine Epitope Mapping Based on Phage Display and Extensive Mutagenesis of the Target Antigen
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    Chapter 28 Epitope Mapping with Random Phage Display Library
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    Chapter 29 Epitope Mapping of Monoclonal and Polyclonal Antibodies Using Bacterial Cell Surface Display
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    Chapter 30 Ion Exchange-High-Performance Liquid Chromatography (IEX-HPLC)
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    Chapter 31 Size Exclusion-High-Performance Liquid Chromatography (SEC-HPLC)
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    Chapter 32 N-Glycosylation Characterization by Liquid Chromatography with Mass Spectrometry
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    Chapter 33 Fc Engineering of Antibodies and Antibody Derivatives by Primary Sequence Alteration and Their Functional Characterization
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    Chapter 34 Labeling and Use of Monoclonal Antibodies in Immunofluorescence: Protocols for Cytoskeletal and Nuclear Antigens
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    Chapter 35 Generation and Use of Antibody Fragments for Structural Studies of Proteins Refractory to Crystallization
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    Chapter 36 Antibody array generation and use.