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Protein Affinity Tags

Overview of attention for book
Cover of 'Protein Affinity Tags'

Table of Contents

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    Book Overview
  2. Altmetric Badge
    Chapter 1 1D4: A Versatile Epitope Tag for the Purification and Characterization of Expressed Membrane and Soluble Proteins
  3. Altmetric Badge
    Chapter 2 Affinity Purification of Heme-Tagged Proteins
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    Chapter 3 Purification of a recombinant protein with cellulose-binding module 3 as the affinity tag.
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    Chapter 4 Purification of E. coli Proteins Using a Self-Cleaving Chitin-Binding Affinity Tag
  6. Altmetric Badge
    Chapter 5 Simplified Protein Purification Using an Autoprocessing, Inducible Enzyme Tag
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    Chapter 6 SUMO as a Solubility Tag and In Vivo Cleavage of SUMO Fusion Proteins with Ulp1.
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    Chapter 7 Rescuing Aggregation-Prone Proteins in Escherichia coli with a Dual His6-MBP Tag.
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    Chapter 8 Expression, Purification, and Immobilization of Recombinant Tamavidin 2 Fusion Proteins
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    Chapter 9 Use of Tandem Affinity Chromatography for Purification of Cannabinoid Receptor CB 2
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    Chapter 10 Detection of protein-protein interactions using tandem affinity purification.
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    Chapter 11 An Improved In Vivo Biotinylation Strategy Combined with FLAG and Antibody Based Approaches for Affinity Purification of Protein Complexes in Mouse Embryonic Stem Cells
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    Chapter 12 Purification of Recombinant Proteins with a Multifunctional GFP Tag
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    Chapter 13 Targeted Purification of SnAvi-Tagged Proteins
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    Chapter 14 An efficient fluorescent protein-based multifunctional affinity purification approach in Mammalian cells.
  16. Altmetric Badge
    Chapter 15 Bimolecular Affinity Purification: A Variation of TAP with Multiple Applications
Attention for Chapter 6: SUMO as a Solubility Tag and In Vivo Cleavage of SUMO Fusion Proteins with Ulp1.
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About this Attention Score

  • In the top 25% of all research outputs scored by Altmetric
  • High Attention Score compared to outputs of the same age (89th percentile)
  • High Attention Score compared to outputs of the same age and source (95th percentile)

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Citations

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Chapter title
SUMO as a Solubility Tag and In Vivo Cleavage of SUMO Fusion Proteins with Ulp1.
Chapter number 6
Book title
Protein Affinity Tags
Published in
Methods in molecular biology, January 2014
DOI 10.1007/978-1-4939-1034-2_6
Pubmed ID
24943315
Book ISBNs
978-1-4939-1033-5, 978-1-4939-1034-2
Authors

Dennis Kuo, Minghua Nie, Albert J Courey, Albert J. Courey, Kuo, Dennis, Nie, Minghua, Courey, Albert J.

Abstract

Expression of proteins in E. coli is often plagued by insolubility of the protein of interest. A solution to this problem is the expression of proteins as fusions to solubility tags such as the SUMO protein. SUMO fusion proteins can be cleaved to remove the SUMO moiety using SUMO-specific proteases such as Ulp1. Here, we describe the use of vectors for the expression of recombinant proteins in E. coli as fusions to the Drosophila SUMO protein. This includes a vector that encodes not only the SUMO tagged protein of interest but also SUMO-tagged Ulp1. Coexpression of these two proteins results in the in vivo cleavage of the protein of interest from the SUMO tag, while still leaving the protein of interest in a form that can be purified from a soluble cell lysate by nickel affinity chromatography.

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X Demographics

X Demographics

The data shown below were collected from the profiles of 2 X users who shared this research output. Click here to find out more about how the information was compiled.
 Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 106 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 106 100%

Demographic breakdown

Readers by professional status Count As %
Student > Bachelor 22 21%
Student > Master 16 15%
Student > Ph. D. Student 16 15%
Researcher 9 8%
Student > Doctoral Student 3 3%
Other 6 6%
Unknown 34 32%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 47 44%
Agricultural and Biological Sciences 10 9%
Immunology and Microbiology 3 3%
Unspecified 2 2%
Chemical Engineering 2 2%
Other 6 6%
Unknown 36 34%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 12. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 01 September 2022.
All research outputs
#2,807,861
of 23,577,654 outputs
Outputs from Methods in molecular biology
#538
of 13,410 outputs
Outputs of similar age
#33,410
of 309,121 outputs
Outputs of similar age from Methods in molecular biology
#26
of 582 outputs
Altmetric has tracked 23,577,654 research outputs across all sources so far. Compared to these this one has done well and is in the 88th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 13,410 research outputs from this source. They receive a mean Attention Score of 3.4. This one has done particularly well, scoring higher than 95% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 309,121 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 89% of its contemporaries.
We're also able to compare this research output to 582 others from the same source and published within six weeks on either side of this one. This one has done particularly well, scoring higher than 95% of its contemporaries.

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