Chapter title |
A “Dock and Lock” Approach to Preparation of Targeted Liposomes
|
---|---|
Chapter number | 7 |
Book title |
Liposomes
|
Published in |
Methods in molecular biology, January 2017
|
DOI | 10.1007/978-1-4939-6591-5_7 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6589-2, 978-1-4939-6591-5
|
Authors |
Marina V. Backer, Joseph M. Backer, Backer, Marina V., Backer, Joseph M. |
Abstract |
We developed a strategy for covalent coupling of targeting proteins to liposomes decorated with a standard adapter protein. This strategy is based on "dock and lock" interactions between two mutated fragments of human RNase I, a 1-15 aa fragment with the R4C amino acid substitution (Cys-tag), and a 21-127-aa fragment with the V118C substitution, (Ad-C). Upon binding to each other, Cys-tag and Ad-C spontaneously form a disulfide bond between the complementary 4C and 118C residues. Therefore, any targeting protein expressed with Cys-tag can be easily coupled to liposomes decorated with Ad-C. Here we describe the preparation of Ad-liposomes followed by coupling them to two Cys-tagged targeted proteins, human vascular endothelial growth factor expressed with N-terminal Cys-tag and a 254-aa long N-terminal fragment of anthrax lethal factor carrying C-terminal Cys-tag. Both proteins retain functional activity after coupling to Ad-C-decorated drug-loaded liposomes. We expect that our "dock and lock" strategy will open new opportunities for development of targeted therapeutic liposomes for research and clinical use. |
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Geographical breakdown
Country | Count | As % |
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Unknown | 5 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Ph. D. Student | 1 | 20% |
Other | 1 | 20% |
Student > Master | 1 | 20% |
Unknown | 2 | 40% |
Readers by discipline | Count | As % |
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Materials Science | 1 | 20% |
Chemistry | 1 | 20% |
Unknown | 3 | 60% |