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Chapter title |
Quantitative Analysis of Protein-RNA Interactions by Gel Mobility Shift
|
---|---|
Chapter number | 7 |
Book title |
RNA-Protein Interaction Protocols
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Published in |
Methods in molecular biology, January 2008
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DOI | 10.1007/978-1-60327-475-3_7 |
Pubmed ID | |
Book ISBNs |
978-1-58829-419-7, 978-1-60327-475-3
|
Authors |
Sean P. Ryder, Michael I. Recht, James R. Williamson, Ryder, Sean P., Recht, Michael I., Williamson, James R. |
Abstract |
The gel mobility shift assay is routinely used to visualize protein-RNA interactions. Its power resides in the ability to resolve free from bound RNA with high resolution in a gel matrix. We review the quantitative application of this approach to elucidate thermodynamic properties of protein-RNA complexes. Assay designs for titration, competition, and stoichiometry experiments are presented for two unrelated model complexes. |
Mendeley readers
The data shown below were compiled from readership statistics for 211 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
United States | 3 | 1% |
United Kingdom | 1 | <1% |
Korea, Republic of | 1 | <1% |
Unknown | 206 | 98% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 65 | 31% |
Researcher | 31 | 15% |
Student > Master | 23 | 11% |
Student > Bachelor | 17 | 8% |
Student > Doctoral Student | 13 | 6% |
Other | 29 | 14% |
Unknown | 33 | 16% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 73 | 35% |
Agricultural and Biological Sciences | 68 | 32% |
Chemistry | 10 | 5% |
Medicine and Dentistry | 4 | 2% |
Immunology and Microbiology | 3 | 1% |
Other | 15 | 7% |
Unknown | 38 | 18% |