Chapter 1 HSV-1 Biology and Life Cycle

Chapter 2 Herpes Simplex Virus Growth, Preparation, and Assay

Chapter 3 Isolation of Herpes Simplex Virus Nucleocapsid DNA

Chapter 4 Construction and Characterization of Bacterial Artificial Chromosomes (BACs) Containing Herpes Simplex Virus Full-Length Genomes

Chapter 5 Engineering HSV-1 Vectors for Gene Therapy.

Chapter 6 Herpes Simplex Virus Type 1 (HSV-1)-Derived Amplicon Vectors

Chapter 7 HSV-1 Amplicon Vectors as Genetic Vaccines

Chapter 8 Modification of HSV-1 to an Oncolytic Virus

Chapter 9 Herpes Simplex Virus Mutant Generation and Dual-Detection Methods for Gaining Insight into Latent/Lytic Cycles In Vivo

Chapter 10 Phenotypic and Genotypic Testing of HSV-1 Resistance to Antivirals

Chapter 11 Using Homogeneous Primary Neuron Cultures to Study Fundamental Aspects of HSV-1 Latency and Reactivation

Chapter 12 Characterization of Extracellular HSV-1 Virions by Proteomics

Chapter 13 A Precipitation-Based Assay to Analyze Interactions of Viral Particles with Cytosolic Host Factors

Chapter 14 Affinity purification combined with mass spectrometry to identify herpes simplex virus protein-protein interactions.

Chapter 15 Preparation of Herpes Simplex Virus-Infected Primary Neurons for Transmission Electron Microscopy

Chapter 16 Multifluorescence Live Analysis of Herpes Simplex Virus Type-1 Replication

Chapter 17 Expression, Purification, and Crystallization of HSV-1 Glycoproteins for Structure Determination

Chapter 18 Cryo-EM Techniques to Resolve the Structure of HSV-1 Capsid-Associated Components

Chapter 19 In Vivo HSV-1 DNA Transport Studies Using Murine Retinal Ganglion Cells

Chapter 20 HSV-1 Protein Expression Using Recombinant Baculoviruses

Chapter 21 The Murine Intravaginal HSV-2 Challenge Model for Investigation of DNA Vaccines