| Chapter title |
Drosophila Drosophila Model for Studying PhagocytosisPhagocytosis Following Neuronal Cell DeathNeuronal Cell Death.
|
|---|---|
| Chapter number | 26 |
| Book title |
Neuronal Cell Death
|
| Published in |
Methods in molecular biology, January 2015
|
| DOI | 10.1007/978-1-4939-2152-2_26 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-2151-5, 978-1-4939-2152-2
|
| Authors |
Boris Shklyar, Flonia Levy-Adam, Estee Kurant, Shklyar, Boris, Levy-Adam, Flonia, Kurant, Estee |
| Abstract |
During central nervous systemcentral nervous system (CNSCNS ) development,development a large number of neuronsneurons die by apoptosisapoptosis and are efficiently removed through phagocytosisphagocytosis . Since apoptosis and apoptotic cell clearanceclearance are highly conserved in evolutionevolution , relatively simple and easily accessible Drosophila Drosophila embryonicembryonic CNS provides a good model to study molecular and cellular mechanisms of these processes. Here, we describe how to assess neuronal apoptosis and glialglial phagocytosis of apoptotic neuronsapoptotic neurons using immunohistochemistryimmunohistochemistry of whole fixedfixed embryoswhole fixed embryos and live imaginglive imaging of developing embryonic CNS. Combination of these different strategies allows a comprehensive analysis of neuronal cell deathneuronal cell death in vivoin vivo . |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 3 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 2 | 67% |
| Lecturer > Senior Lecturer | 1 | 33% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 1 | 33% |
| Agricultural and Biological Sciences | 1 | 33% |
| Neuroscience | 1 | 33% |