Chapter title |
Generation of Infectious Prions and Detection with the Prion-Infected Cell Assay
|
---|---|
Chapter number | 9 |
Book title |
Prions
|
Published in |
Methods in molecular biology, January 2017
|
DOI | 10.1007/978-1-4939-7244-9_9 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7242-5, 978-1-4939-7244-9
|
Authors |
Laura J. Vella, Bradley Coleman, Andrew F. Hill, Vella, Laura J., Coleman, Bradley, Hill, Andrew F. |
Abstract |
Cell lines propagating prions are an efficient and useful means for studying the cellular and molecular mechanisms implicated in prion disease. Utilization of cell-based models has led to the finding that PrP(C) and PrP(Sc) are released from cells in association with extracellular vesicles known as exosomes. Exosomes have been shown to act as vehicles for infectivity, transferring infectivity between cell lines and providing a mechanism for prion spread between tissues. Here, we describe the methods for generating a prion-propagating cell line with prion-infected brain homogenate, cell lysate, conditioned media, and exosomes and also detection of protease-resistant PrP with the prion-infected cell assay. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 15 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 4 | 27% |
Student > Bachelor | 2 | 13% |
Student > Doctoral Student | 2 | 13% |
Unspecified | 1 | 7% |
Other | 1 | 7% |
Other | 1 | 7% |
Unknown | 4 | 27% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 3 | 20% |
Biochemistry, Genetics and Molecular Biology | 3 | 20% |
Medicine and Dentistry | 2 | 13% |
Unspecified | 1 | 7% |
Immunology and Microbiology | 1 | 7% |
Other | 1 | 7% |
Unknown | 4 | 27% |