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Tissue Proteomics

Overview of attention for book
Cover of 'Tissue Proteomics'

Table of Contents

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    Book Overview
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    Chapter 73 Depletion of Myofibril-Associated Proteins Using Selective Protein Extraction as a Tool in Cardiac Proteomics
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    Chapter 74 Untargeted Screening of Urinary Peptides Using Offline Nano-Liquid Chromatography: MALDI-TOF/TOF Mass Spectrometry
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    Chapter 75 Identifying Clinically Relevant Proteins for Targeted Analysis in the Development of a Multiplexed Proteomic Biomarker Assay
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    Chapter 76 GeLC-MS: A Sample Preparation Method for Proteomics Analysis of Minimal Amount of Tissue
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    Chapter 77 Isobaric Labeling-Based LC-MS/MS Strategy for Comprehensive Profiling of Human Pancreatic Tissue Proteome
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    Chapter 78 Quantitative Proteomic Analysis of Mass Limited Tissue Samples for Spatially Resolved Tissue Profiling
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    Chapter 79 HLA Class I and Class II-Induced Intracellular Signaling and Molecular Associations in Primary Human Endothelial Cells
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    Chapter 80 Differential Adipose Tissue Proteomics
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    Chapter 81 MALDI Imaging Mass Spectrometry of N-glycans and Tryptic Peptides from the Same Formalin-Fixed, Paraffin-Embedded Tissue Section
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    Chapter 82 Straightforward Protocol for Gel-Free Proteomic Analysis of Adipose Tissue
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    Chapter 85 Characterization of Protein Complexes Using Chemical Cross-Linking Coupled Electrospray Mass Spectrometry
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    Chapter 86 In Situ Hybridization and Double Immunohistochemistry for the Detection of VEGF-A mRNA and CD34/Collagen IV Proteins in Renal Transplant Biopsies
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    Chapter 87 High-Throughput Proteomic Analysis of Fresh-Frozen Biopsy Tissue Samples Using Pressure Cycling Technology Coupled with SWATH Mass Spectrometry
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    Chapter 88 Combination Strategy of Quantitative Proteomics Uncovers the Related Proteins of Colorectal Cancer in the Interstitial Fluid of Colonic Tissue from the AOM-DSS Mouse Model
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    Chapter 89 Purification of Target Proteins from Native Tissues: CCT Complex from Bovine Testes and PP2Ac from Porcine Brains
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    Chapter 90 Optimization for Peptide Sample Preparation for Urine Peptidomics
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    Chapter 91 Fractionation of Soluble Proteins Using DEAE-Sepharose, SP-Sepharose, and Phenyl Sepharose Chromatographies for Proteomics
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    Chapter 92 Discovery of Immune Reactive Human Proteins by High-Density Protein Arrays and Customized Validation of Potential Biomarkers by ELISA
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    Chapter 93 LC-SRM-Based Targeted Quantification of Urinary Protein Biomarkers
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    Chapter 94 Integrative Analysis of Proteomics Data to Obtain Clinically Relevant Markers
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    Chapter 111 Targeted Proteomics Driven Verification of Biomarker Candidates Associated with Breast Cancer Aggressiveness
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    Chapter 112 Multiple Reaction Monitoring Using Double Isotopologue Peptide Standards for Protein Quantification
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    Chapter 113 Quantification of Breast Cancer Protein Biomarkers at Different Expression Levels in Human Tumors
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    Chapter 114 MALDI Imaging Combined with Laser Microdissection-Based Microproteomics for Protein Identification: Application to Intratumor Heterogeneity Studies
Attention for Chapter 81: MALDI Imaging Mass Spectrometry of N-glycans and Tryptic Peptides from the Same Formalin-Fixed, Paraffin-Embedded Tissue Section
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Chapter title
MALDI Imaging Mass Spectrometry of N-glycans and Tryptic Peptides from the Same Formalin-Fixed, Paraffin-Embedded Tissue Section
Chapter number 81
Book title
Tissue Proteomics
Published in
Methods in molecular biology, October 2017
DOI 10.1007/7651_2017_81
Pubmed ID
Book ISBNs
978-1-4939-7852-6, 978-1-4939-7854-0
Authors

Peggi M. Angel, Anand Mehta, Kim Norris-Caneda, Richard R. Drake, Angel, Peggi M., Mehta, Anand, Norris-Caneda, Kim, Drake, Richard R.

Abstract

Matrix-assisted laser desorption/ionization (MALDI) imaging mass spectrometry (IMS) is a unique and well developed tool for probing the protein content of formalin-fixed, paraffin-embedded tissue (FFPE). Integral to this approach is the application of trypsin, and more recently peptide N-glycosidase F, to release tryptic peptides or N-glycans from tissue and report localization of distinct species. This is typically done on serial or adjacent tissue sections, and there is an emerging need to understand the colocalized protein population linked to the exact same regions of N-glycans. Here we describe an approach where N-glycans are first imaged from a tissue section followed by reprocessing of the same tissue section for tryptic peptide MALDI IMS. Strategies for colocalizing peptides to target N-glycans or N-glycan regions are described.

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 43 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 43 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 9 21%
Student > Master 4 9%
Student > Bachelor 4 9%
Researcher 3 7%
Student > Postgraduate 3 7%
Other 9 21%
Unknown 11 26%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 11 26%
Chemistry 6 14%
Medicine and Dentistry 4 9%
Agricultural and Biological Sciences 4 9%
Pharmacology, Toxicology and Pharmaceutical Science 3 7%
Other 3 7%
Unknown 12 28%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 24 October 2017.
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#18,574,814
of 23,006,268 outputs
Outputs from Methods in molecular biology
#7,961
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Outputs of similar age
#250,789
of 327,563 outputs
Outputs of similar age from Methods in molecular biology
#25
of 34 outputs
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