Patch-Clamp Methods and Protocols

Chapter 1 History of Electrophysiology and the Patch Clamp

Chapter 2 Whole-Cell Patch-Clamp Analysis of Recombinant NMDA Receptor Pharmacology Using Brief Glutamate Applications

Chapter 3 Patch Clamp Combined with Voltage/Concentration Clamp to Determine the Kinetics and Voltage Dependency of N -Methyl- d -aspartate (NMDA) Receptor Open Channel Blockers

Chapter 4 State-of-the-Art Automated Patch Clamp: Heat Activation, Action Potentials, and High Throughput in Ion Channel Screening

Chapter 5 Industrializing Electrophysiology: HT Automated Patch Clamp on SyncroPatch ® 96 Using Instant Frozen Cells

Chapter 6 Planar Patch Clamp for Neuronal Networks—Considerations and Future Perspectives

Chapter 7 Methods for the Study of Synaptic Receptor Functional Properties

Chapter 8 Single-Cell RT-PCR, a Technique to Decipher the Electrical, Anatomical, and Genetic Determinants of Neuronal Diversity

Chapter 9 Whole-Cell Voltage Clamp on Skeletal Muscle Fibers with the Silicone-Clamp Technique

Chapter 10 Multiphoton Imaging Approaches for Studying Striatal Dendritic Excitability

Chapter 11 Investigation of Synaptic Microcircuits Using Patch-Clamp Paired Recordings in Acute Brain Slices

Chapter 12 Outside-Out “Sniffer-Patch” Clamp Technique for In Situ Measures of Neurotransmitter Release

Chapter 13 A Cost-Effective Method for Preparing, Maintaining, and Transfecting Neurons in Organotypic Slices

Chapter 14 Acute Brain Slice Methods for Adult and Aging Animals: Application of Targeted Patch Clamp Analysis and Optogenetics

Chapter 15 Synaptic Connectivity in Engineered Neuronal Networks

Chapter 17 Whole-Cell Patch-Clamp Recordings in Freely Moving Animals

Chapter 18 Pressure-Polished Borosilicate Pipettes are “Universal Sealer” Yielding Low Access Resistance and Efficient Intracellular Perfusion

Chapter 19 Current Recordings at the Single Channel Level in Adult Mammalian Isolated Cardiomyocytes

Chapter 20 Patch-Clamp Methods and Protocols

Chapter 21 Dynamic Clamp in Cardiac and Neuronal Systems Using RTXI

Chapter 22 Ion Selectivity of Pore-Forming Peptides and Ion Channels Measured in Xenopus Oocytes

Chapter 23 Principles of Single-Channel Kinetic Analysis