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Antiviral RNAi

Overview of attention for book
Cover of 'Antiviral RNAi'

Table of Contents

  1. Altmetric Badge
    Book Overview
  2. Altmetric Badge
    Chapter 1 Defense and Counterdefense in the RNAi-Based Antiviral Immune System in Insects
  3. Altmetric Badge
    Chapter 2 RNAi and Cellular miRNAs in Infections by Mammalian Viruses
  4. Altmetric Badge
    Chapter 3 Viral miRNAs
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    Chapter 4 Progress in RNAi-Based Antiviral Therapeutics
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    Chapter 5 Chemical Modification of Small Interfering RNA
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    Chapter 6 Viral Small RNA Cloning and Sequencing
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    Chapter 7 Visitor, an informatic pipeline for analysis of viral siRNA sequencing datasets.
  9. Altmetric Badge
    Chapter 8 Computational prediction of viral miRNAs.
  10. Altmetric Badge
    Chapter 9 Antiviral RNAi
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    Chapter 10 Detection of viral microRNA with S1 nuclease protection assay.
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    Chapter 11 Characterization of RISC-Associated Adenoviral Small RNAs
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    Chapter 12 Identification of Viral Suppressors of RNAi by a Reporter Assay in Drosophila S2 Cell Culture
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    Chapter 13 Dicer Assay in Drosophila S2 Cell Extract
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    Chapter 14 Slicer Activity in Drosophila melanogaster S2 Extract
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    Chapter 15 Gel Mobility Shift Assays for RNA Binding Viral RNAi Suppressors
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    Chapter 16 dsRNA Uptake in Adult Drosophila
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    Chapter 17 Design of Small Interfering RNAs for Antiviral Applications
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    Chapter 18 RNAi-inducing lentiviral vectors for anti-HIV-1 gene therapy.
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    Chapter 19 Production of Multicopy shRNA Lentiviral Vectors for Antiviral Therapy
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    Chapter 20 Intranasal Delivery of Antiviral siRNA
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    Chapter 21 Antibody-Mediated Delivery of siRNAs for Anti-HIV Therapy
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    Chapter 22 Aptamer-Targeted RNAi for HIV-1 Therapy
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    Chapter 23 RNAi Screening for Host Factors Involved in Viral Infection Using Drosophila Cells
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    Chapter 24 Genome-Wide RNAi Screen for Viral Replication in Mammalian Cell Culture
  26. Altmetric Badge
    Chapter 25 RNAi Screening in Mammalian Cells to Identify Novel Host Cell Molecules Involved in the Regulation of Viral Infections
  27. Altmetric Badge
    Chapter 26 Production of Multicopy shRNA Lentiviral Vectors for Antiviral Therapy
Attention for Chapter 10: Detection of viral microRNA with S1 nuclease protection assay.
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Chapter title
Detection of viral microRNA with S1 nuclease protection assay.
Chapter number 10
Book title
Antiviral RNAi
Published in
Methods in molecular biology, January 2011
DOI 10.1007/978-1-61779-037-9_10
Pubmed ID
Book ISBNs
978-1-61779-036-2, 978-1-61779-037-9
Authors

Matthias John, Sébastien Pfeffer

Abstract

Mammalian host cells and their viral pathogens express and make use of short noncoding RNA molecules to control the infectious cycle. In order to understand their physiological role, it is necessary to develop tools for detection and quantification of these molecules. Here, we present a simple, specific, and very sensitive protocol using short radioactive DNA oligonucleotides for hybridization to homologous RNA target in a nuclease protection assay. The S1 nuclease from Aspergillus oryzae degrades single-stranded oligonucleotides composed of either deoxynucleotides or ribonucleotides. In contrast, double-stranded DNA, double-stranded RNA, or DNA-RNA hybrids are resistant to digestion. Subsequent analysis of the protected DNA oligonucleotide with denaturing gel electrophoresis results in radioactive signals strictly proportional to the abundance of short RNA in a given sample. The protocol works equally well for in vitro cell culture assays and for tissue samples obtained from in vivo experiments.

Twitter Demographics

The data shown below were collected from the profile of 1 tweeter who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 12 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
France 1 8%
Unknown 11 92%

Demographic breakdown

Readers by professional status Count As %
Researcher 4 33%
Student > Bachelor 2 17%
Professor 2 17%
Professor > Associate Professor 2 17%
Other 1 8%
Other 1 8%
Readers by discipline Count As %
Agricultural and Biological Sciences 6 50%
Veterinary Science and Veterinary Medicine 1 8%
Computer Science 1 8%
Biochemistry, Genetics and Molecular Biology 1 8%
Immunology and Microbiology 1 8%
Other 2 17%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 07 July 2011.
All research outputs
#3,108,445
of 4,506,407 outputs
Outputs from Methods in molecular biology
#1,460
of 3,194 outputs
Outputs of similar age
#2,400,203
of 3,441,809 outputs
Outputs of similar age from Methods in molecular biology
#1,235
of 2,699 outputs
Altmetric has tracked 4,506,407 research outputs across all sources so far. This one is in the 21st percentile – i.e., 21% of other outputs scored the same or lower than it.
So far Altmetric has tracked 3,194 research outputs from this source. They receive a mean Attention Score of 1.4. This one is in the 41st percentile – i.e., 41% of its peers scored the same or lower than it.
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We're also able to compare this research output to 2,699 others from the same source and published within six weeks on either side of this one. This one is in the 41st percentile – i.e., 41% of its contemporaries scored the same or lower than it.