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Antiviral RNAi

Overview of attention for book
Cover of 'Antiviral RNAi'

Table of Contents

  1. Altmetric Badge
    Book Overview
  2. Altmetric Badge
    Chapter 1 Defense and Counterdefense in the RNAi-Based Antiviral Immune System in Insects
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    Chapter 2 RNAi and Cellular miRNAs in Infections by Mammalian Viruses
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    Chapter 3 Viral miRNAs
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    Chapter 4 Progress in RNAi-Based Antiviral Therapeutics
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    Chapter 5 Chemical Modification of Small Interfering RNA
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    Chapter 6 Viral Small RNA Cloning and Sequencing
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    Chapter 7 Visitor, an informatic pipeline for analysis of viral siRNA sequencing datasets.
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    Chapter 8 Computational prediction of viral miRNAs.
  10. Altmetric Badge
    Chapter 9 Antiviral RNAi
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    Chapter 10 Detection of viral microRNA with S1 nuclease protection assay.
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    Chapter 11 Characterization of RISC-Associated Adenoviral Small RNAs
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    Chapter 12 Identification of Viral Suppressors of RNAi by a Reporter Assay in Drosophila S2 Cell Culture
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    Chapter 13 Dicer Assay in Drosophila S2 Cell Extract
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    Chapter 14 Slicer Activity in Drosophila melanogaster S2 Extract
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    Chapter 15 Gel Mobility Shift Assays for RNA Binding Viral RNAi Suppressors
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    Chapter 16 dsRNA Uptake in Adult Drosophila
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    Chapter 17 Design of Small Interfering RNAs for Antiviral Applications
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    Chapter 18 RNAi-inducing lentiviral vectors for anti-HIV-1 gene therapy.
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    Chapter 19 Production of Multicopy shRNA Lentiviral Vectors for Antiviral Therapy
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    Chapter 20 Intranasal Delivery of Antiviral siRNA
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    Chapter 21 Antibody-Mediated Delivery of siRNAs for Anti-HIV Therapy
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    Chapter 22 Aptamer-Targeted RNAi for HIV-1 Therapy
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    Chapter 23 RNAi Screening for Host Factors Involved in Viral Infection Using Drosophila Cells
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    Chapter 24 Genome-Wide RNAi Screen for Viral Replication in Mammalian Cell Culture
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    Chapter 25 RNAi Screening in Mammalian Cells to Identify Novel Host Cell Molecules Involved in the Regulation of Viral Infections
  27. Altmetric Badge
    Chapter 26 Production of Multicopy shRNA Lentiviral Vectors for Antiviral Therapy
Attention for Chapter 7: Visitor, an informatic pipeline for analysis of viral siRNA sequencing datasets.
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Chapter title
Visitor, an informatic pipeline for analysis of viral siRNA sequencing datasets.
Chapter number 7
Book title
Antiviral RNAi
Published in
Methods in molecular biology, March 2011
DOI 10.1007/978-1-61779-037-9_7
Pubmed ID
Book ISBNs
978-1-61779-036-2, 978-1-61779-037-9
Authors

Antoniewski C, Christophe Antoniewski

Abstract

High-throughput sequencing emerged as a powerful approach to characterize siRNA populations -generated by hosts in response to viral infections. Here we described an informatic pipeline visitor to analyze in-house large sequencing datasets generated from Illumina sequencing of Drosophila small RNA libraries. The visitor perl script is designed to treat fastq sequence datasets from the Illumina sequencing platform, using a computer running under a UNIX compliant operating system (MacOS X, Linux, etc.). visitor first generates a detailed report of the sequence quality of the Illumina run. Then, using the Novoalign software, the script removes reads that match with the D. melanogaster genome from the sequencing data set. The remaining reads are aligned to a viral reference library, which can contain one or several virus genomes. visitor provides a hit table of identified viral siRNAs as well as graphics eps files of viral siRNA profiles. Unmatched small RNAs are also available in a fast format for de novo assembly and new virus discovery.

Mendeley readers

The data shown below were compiled from readership statistics for 13 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 13 100%

Demographic breakdown

Readers by professional status Count As %
Student > Bachelor 3 23%
Professor > Associate Professor 2 15%
Researcher 2 15%
Student > Master 2 15%
Professor 1 8%
Other 1 8%
Unknown 2 15%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 4 31%
Agricultural and Biological Sciences 4 31%
Social Sciences 1 8%
Medicine and Dentistry 1 8%
Engineering 1 8%
Other 0 0%
Unknown 2 15%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 11 April 2011.
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#2,904,032
of 3,628,302 outputs
Outputs from Methods in molecular biology
#1,443
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Outputs of similar age
#167,833
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Outputs of similar age from Methods in molecular biology
#127
of 268 outputs
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