| Chapter title |
Random Spore Analysis in Fission Yeast
|
|---|---|
| Chapter number | 17 |
| Book title |
Schizosaccharomyces pombe
|
| Published in |
Methods in molecular biology, January 2018
|
| DOI | 10.1007/978-1-4939-7546-4_17 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-7545-7, 978-1-4939-7546-4
|
| Authors |
Wilber Escorcia, Susan L. Forsburg, Escorcia, Wilber, Forsburg, Susan L. |
| Abstract |
Random spore analysis (RSA) is a tool that allows for the screening of a large number of meiotic products. It requires only a limited effort, and is often the method of choice for constructing strains with unambiguous genotypes. It is also useful to identify the frequency of rare events. Strains are crossed on a nitrogen-limiting medium for three days. Mated cells are observed under the microscope to check for the presence of ripe asci. To release spores from their ascus, a sample of the cross is taken from the mating plate and resuspended in an enzyme solution overnight at 25-29 °C. Spores are then counted using a hemocytometer before plating an appropriate number. Incubation at the appropriate temperature follows until colonies form. |
X Demographics
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| France | 1 | 50% |
| Japan | 1 | 50% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 2 | 100% |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 10 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 3 | 30% |
| Student > Postgraduate | 2 | 20% |
| Student > Ph. D. Student | 1 | 10% |
| Student > Master | 1 | 10% |
| Student > Bachelor | 1 | 10% |
| Other | 0 | 0% |
| Unknown | 2 | 20% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 7 | 70% |
| Agricultural and Biological Sciences | 1 | 10% |
| Unknown | 2 | 20% |