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Schizosaccharomyces pombe

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Cover of 'Schizosaccharomyces pombe'

Table of Contents

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    Book Overview
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    Chapter 1 Preparation of Solutions and Reagents
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    Chapter 2 Analysis of Fission Yeast Single DNA Molecules on the Megabase Scale Using DNA Combing
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    Chapter 3 Chromatin Immunoprecipitation-Polymerase Chain Reaction (ChIP-PCR) Detects Methylation, Acetylation, and Ubiquitylation in S. pombe
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    Chapter 4 Primer Design and Inverse PCR on Yeast Display Antibody Selection Outputs
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    Chapter 5 Molecular Cloning and Characterization of Small Viral Genome in Fission Yeast
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    Chapter 6 Total RNA Isolation and Quantification of Specific RNAs in Fission Yeast
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    Chapter 7 Analysis of Reverse Transcribed mRNA Using PCR and Polyacrylamide Gel Electrophoresis
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    Chapter 8 The No-Nonsens SDS-PAGE
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    Chapter 9 Crystallization of Recombinant α-Actinin and Related Proteins
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    Chapter 10 Estimation of GFP-Nucleoporin Amount Based on Fluorescence Microscopy
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    Chapter 11 Antibody Pull-Down Experiments in Fission Yeast
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    Chapter 12 Preparation of Cell Lysates of Fission Yeast for Immunoprecipitation
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    Chapter 13 Wide-band Electrical Impedance Spectroscopy (EIS) Measures S. pombe Cell Growth in vivo
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    Chapter 14 In Situ Chromatin-Binding Assay Using Epifluorescent Microscopy in S. pombe
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    Chapter 15 High-Frequency Lithium Acetate Transformation of Schizosaccharomyces pombe
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    Chapter 16 Tetrad Dissection in Fission Yeast
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    Chapter 17 Random Spore Analysis in Fission Yeast
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    Chapter 18 Duplication and Transformation of the Schizosaccharomyces pombe Collection of Deletion Strains
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    Chapter 19 Schizosaccharomyces pombe Biotechnological Applications in Winemaking
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    Chapter 20 Schizosaccharomyces pombe Isolation Protocol
Attention for Chapter 14: In Situ Chromatin-Binding Assay Using Epifluorescent Microscopy in S. pombe
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Chapter title
In Situ Chromatin-Binding Assay Using Epifluorescent Microscopy in S. pombe
Chapter number 14
Book title
Schizosaccharomyces pombe
Published in
Methods in molecular biology, January 2018
DOI 10.1007/978-1-4939-7546-4_14
Pubmed ID
Book ISBNs
978-1-4939-7545-7, 978-1-4939-7546-4
Authors

Jinpu Yang, Fei Li, Yang, Jinpu, Li, Fei

Abstract

Chromatin-associated proteins play critical roles in many cellular processes, including gene expression, epigenetic regulation, DNA repair, recombination, and replication. Especially, epigenetic landscape, shaped by a variety of chromatin-binding proteins, is dynamic and regulated in a context-dependent manner. In situ chromatin-binding assay is a powerful but simple tool to investigate how proteins, such as epigenetic components, associate with chromatin. This approach relies on the fact that chromatin bound proteins are more resistant to detergent extraction. Here, we describe a protocol for the in situ chromatin-binding assay used in Schizosaccaromyces pombe.

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