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Chapter title |
In Situ Chromatin-Binding Assay Using Epifluorescent Microscopy in S. pombe
|
---|---|
Chapter number | 14 |
Book title |
Schizosaccharomyces pombe
|
Published in |
Methods in molecular biology, January 2018
|
DOI | 10.1007/978-1-4939-7546-4_14 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7545-7, 978-1-4939-7546-4
|
Authors |
Jinpu Yang, Fei Li, Yang, Jinpu, Li, Fei |
Abstract |
Chromatin-associated proteins play critical roles in many cellular processes, including gene expression, epigenetic regulation, DNA repair, recombination, and replication. Especially, epigenetic landscape, shaped by a variety of chromatin-binding proteins, is dynamic and regulated in a context-dependent manner. In situ chromatin-binding assay is a powerful but simple tool to investigate how proteins, such as epigenetic components, associate with chromatin. This approach relies on the fact that chromatin bound proteins are more resistant to detergent extraction. Here, we describe a protocol for the in situ chromatin-binding assay used in Schizosaccaromyces pombe. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
Country | Count | As % |
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France | 1 | 100% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 1 | 100% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 10 February 2018.
All research outputs
#20,465,050
of 23,023,224 outputs
Outputs from Methods in molecular biology
#9,945
of 13,166 outputs
Outputs of similar age
#378,196
of 442,361 outputs
Outputs of similar age from Methods in molecular biology
#1,193
of 1,498 outputs
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So far Altmetric has tracked 13,166 research outputs from this source. They receive a mean Attention Score of 3.4. This one is in the 1st percentile – i.e., 1% of its peers scored the same or lower than it.
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We're also able to compare this research output to 1,498 others from the same source and published within six weeks on either side of this one. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.