Report for: Schizosaccharomyces pombe

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Chapter title	Preparation of Cell Lysates of Fission Yeast for Immunoprecipitation
Chapter number	12
Book title	Schizosaccharomyces pombe
Published in	Methods in molecular biology, January 2018
DOI	10.1007/978-1-4939-7546-4_12
Pubmed ID	29423853
Book ISBNs	978-1-4939-7545-7, 978-1-4939-7546-4
Authors	Akihisa Matsuyama, Atsuko Shirai, Minoru Yoshida, Matsuyama, Akihisa, Shirai, Atsuko, Yoshida, Minoru
Abstract	Immunoprecipitation is one of the most important and widely used techniques for the detection and purification of a protein of interest. Thanks to highly specific interaction between antigen and antibody, a target protein is purified and concentrated effectively. To obtain reasonable amounts of a target protein, it is crucially important to prepare total cell lysates in which the target protein is present in a soluble form. Here, we describe methods to prepare total cell lysates of fission yeast, which are then used directly for immunoprecipitation. We also describe some tips to select reagents for preparing buffers having a substantial impact on protein solubility, because there is essentially no reagent that can accommodate the full range of proteins having different characteristics.

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France	1	100%

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Members of the public	1	100%

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Unspecified	1	25%
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Unspecified	1	25%
Unknown	3	75%

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Schizosaccharomyces pombe

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