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Schizosaccharomyces pombe

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Cover of 'Schizosaccharomyces pombe'

Table of Contents

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    Book Overview
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    Chapter 1 Preparation of Solutions and Reagents
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    Chapter 2 Analysis of Fission Yeast Single DNA Molecules on the Megabase Scale Using DNA Combing
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    Chapter 3 Chromatin Immunoprecipitation-Polymerase Chain Reaction (ChIP-PCR) Detects Methylation, Acetylation, and Ubiquitylation in S. pombe
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    Chapter 4 Primer Design and Inverse PCR on Yeast Display Antibody Selection Outputs
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    Chapter 5 Molecular Cloning and Characterization of Small Viral Genome in Fission Yeast
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    Chapter 6 Total RNA Isolation and Quantification of Specific RNAs in Fission Yeast
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    Chapter 7 Analysis of Reverse Transcribed mRNA Using PCR and Polyacrylamide Gel Electrophoresis
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    Chapter 8 The No-Nonsens SDS-PAGE
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    Chapter 9 Crystallization of Recombinant α-Actinin and Related Proteins
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    Chapter 10 Estimation of GFP-Nucleoporin Amount Based on Fluorescence Microscopy
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    Chapter 11 Antibody Pull-Down Experiments in Fission Yeast
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    Chapter 12 Preparation of Cell Lysates of Fission Yeast for Immunoprecipitation
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    Chapter 13 Wide-band Electrical Impedance Spectroscopy (EIS) Measures S. pombe Cell Growth in vivo
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    Chapter 14 In Situ Chromatin-Binding Assay Using Epifluorescent Microscopy in S. pombe
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    Chapter 15 High-Frequency Lithium Acetate Transformation of Schizosaccharomyces pombe
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    Chapter 16 Tetrad Dissection in Fission Yeast
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    Chapter 17 Random Spore Analysis in Fission Yeast
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    Chapter 18 Duplication and Transformation of the Schizosaccharomyces pombe Collection of Deletion Strains
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    Chapter 19 Schizosaccharomyces pombe Biotechnological Applications in Winemaking
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    Chapter 20 Schizosaccharomyces pombe Isolation Protocol
Attention for Chapter 8: The No-Nonsens SDS-PAGE
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Chapter title
The No-Nonsens SDS-PAGE
Chapter number 8
Book title
Schizosaccharomyces pombe
Published in
Methods in molecular biology, January 2018
DOI 10.1007/978-1-4939-7546-4_8
Pubmed ID
Book ISBNs
978-1-4939-7545-7, 978-1-4939-7546-4
Authors

Lars Backman, Karina Persson, Backman, Lars, Persson, Karina

Abstract

The discontinuous polyacrylamide gel electrophoresis system devised by Laemmli (Nature 227:680-685, 1970) has not only been used in numerous laboratories but has also been modified in several ways since its birth. In our laboratories, we use a modified Laemmli SDS-PAGE system for following protein purification as well as for analysis of certain protein-protein interactions, mainly involving filametous actin.

Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 22 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 22 100%

Demographic breakdown

Readers by professional status Count As %
Student > Bachelor 5 23%
Student > Master 4 18%
Student > Ph. D. Student 3 14%
Other 2 9%
Lecturer > Senior Lecturer 1 5%
Other 0 0%
Unknown 7 32%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 9 41%
Chemical Engineering 1 5%
Pharmacology, Toxicology and Pharmaceutical Science 1 5%
Immunology and Microbiology 1 5%
Medicine and Dentistry 1 5%
Other 1 5%
Unknown 8 36%