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Schizosaccharomyces pombe

Overview of attention for book
Cover of 'Schizosaccharomyces pombe'

Table of Contents

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    Book Overview
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    Chapter 1 Preparation of Solutions and Reagents
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    Chapter 2 Analysis of Fission Yeast Single DNA Molecules on the Megabase Scale Using DNA Combing
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    Chapter 3 Chromatin Immunoprecipitation-Polymerase Chain Reaction (ChIP-PCR) Detects Methylation, Acetylation, and Ubiquitylation in S. pombe
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    Chapter 4 Primer Design and Inverse PCR on Yeast Display Antibody Selection Outputs
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    Chapter 5 Molecular Cloning and Characterization of Small Viral Genome in Fission Yeast
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    Chapter 6 Total RNA Isolation and Quantification of Specific RNAs in Fission Yeast
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    Chapter 7 Analysis of Reverse Transcribed mRNA Using PCR and Polyacrylamide Gel Electrophoresis
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    Chapter 8 The No-Nonsens SDS-PAGE
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    Chapter 9 Crystallization of Recombinant α-Actinin and Related Proteins
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    Chapter 10 Estimation of GFP-Nucleoporin Amount Based on Fluorescence Microscopy
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    Chapter 11 Antibody Pull-Down Experiments in Fission Yeast
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    Chapter 12 Preparation of Cell Lysates of Fission Yeast for Immunoprecipitation
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    Chapter 13 Wide-band Electrical Impedance Spectroscopy (EIS) Measures S. pombe Cell Growth in vivo
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    Chapter 14 In Situ Chromatin-Binding Assay Using Epifluorescent Microscopy in S. pombe
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    Chapter 15 High-Frequency Lithium Acetate Transformation of Schizosaccharomyces pombe
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    Chapter 16 Tetrad Dissection in Fission Yeast
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    Chapter 17 Random Spore Analysis in Fission Yeast
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    Chapter 18 Duplication and Transformation of the Schizosaccharomyces pombe Collection of Deletion Strains
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    Chapter 19 Schizosaccharomyces pombe Biotechnological Applications in Winemaking
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    Chapter 20 Schizosaccharomyces pombe Isolation Protocol
Attention for Chapter 2: Analysis of Fission Yeast Single DNA Molecules on the Megabase Scale Using DNA Combing
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Chapter title
Analysis of Fission Yeast Single DNA Molecules on the Megabase Scale Using DNA Combing
Chapter number 2
Book title
Schizosaccharomyces pombe
Published in
Methods in molecular biology, January 2018
DOI 10.1007/978-1-4939-7546-4_2
Pubmed ID
29423843
Book ISBNs
978-1-4939-7545-7, 978-1-4939-7546-4
Authors

Atanas Kaykov, Paul Nurse, Kaykov, Atanas, Nurse, Paul

Abstract

DNA combing enables the quantitative analysis of DNA replication, DNA recombination, DNA-protein interaction, and DNA methylation along genomic single DNA molecules at 1 kb resolution. However, DNA combing has been restricted to short 200-500 kb long DNA fragments, which introduces significant bias in data analysis. An improved DNA combing methodology that allows to routinely image Mb-scale single DNA molecules and occasionally up to full-length fission yeast chromosomes is presented in this chapter. DNA combing of Mb-scale single DNA molecules can be applied to accurately measure the dynamic properties of DNA replication such as the rate of origin firing, replication fork velocity, fork directionality and the frequency of fork blockage. In addition, Mb-scale single DNA molecules enable the quantitative analysis of complex genomic rearrangements including gross chromosomal translocations, repetitive DNA sequences, large deletions, and duplications, which are difficult to investigate with deep sequencing strategies.

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X Demographics

The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
 Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 5 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 5 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 2 40%
Student > Bachelor 2 40%
Student > Master 1 20%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 2 40%
Agricultural and Biological Sciences 2 40%
Computer Science 1 20%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 19 February 2018.
All research outputs
#19,594,120
of 24,093,053 outputs
Outputs from Methods in molecular biology
#8,407
of 13,601 outputs
Outputs of similar age
#338,972
of 449,849 outputs
Outputs of similar age from Methods in molecular biology
#942
of 1,480 outputs
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So far Altmetric has tracked 13,601 research outputs from this source. They receive a mean Attention Score of 3.5. This one is in the 23rd percentile – i.e., 23% of its peers scored the same or lower than it.
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