| Chapter title |
Wide-band Electrical Impedance Spectroscopy (EIS) Measures S. pombe Cell Growth in vivo
|
|---|---|
| Chapter number | 13 |
| Book title |
Schizosaccharomyces pombe
|
| Published in |
Methods in molecular biology, January 2018
|
| DOI | 10.1007/978-1-4939-7546-4_13 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-7545-7, 978-1-4939-7546-4
|
| Authors |
Zhen Zhu, Olivier Frey, Andreas Hierlemann, Zhu, Zhen, Frey, Olivier, Hierlemann, Andreas |
| Abstract |
This chapter describes a microfluidic device that enables immobilization and culturing of single rod-shaped S. pombe cells in a stand-up mode. The wide-band electrical impedance spectroscopy (EIS) has been integrated in the microfluidic device to continuously measure cell growth of single S. pombe cells. Cell growth curves showing cellular and intracellular features at high spatiotemporal resolution can be obtained from EIS signals. The features include longitudinal cell elongation in the G2 phase, mitosis, and cell division during an entire cell cycle of S. pombe cells. Microfluidics-based EIS systems provide, hence, a tool for dynamic single-cell studies. |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 8 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Lecturer | 2 | 25% |
| Student > Master | 2 | 25% |
| Researcher | 2 | 25% |
| Student > Bachelor | 1 | 13% |
| Unspecified | 1 | 13% |
| Other | 1 | 13% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 2 | 25% |
| Chemical Engineering | 1 | 13% |
| Unspecified | 1 | 13% |
| Agricultural and Biological Sciences | 1 | 13% |
| Physics and Astronomy | 1 | 13% |
| Other | 1 | 13% |
| Unknown | 1 | 13% |