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Plant Epigenetics

Overview of attention for book
Cover of 'Plant Epigenetics'

Table of Contents

  1. Altmetric Badge
    Book Overview
  2. Altmetric Badge
    Chapter 1 Plant Epigenetics
  3. Altmetric Badge
    Chapter 2 Plant Epigenetics
  4. Altmetric Badge
    Chapter 3 Analysis of Locus-Specific Changes in Methylation Patterns Using a COBRA (Combined Bisulfite Restriction Analysis) Assay
  5. Altmetric Badge
    Chapter 4 Detection of Changes in Global Genome Methylation Using the Cytosine-Extension Assay
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    Chapter 5 In Situ Analysis of DNA Methylation in Plants
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    Chapter 6 Analysis of Mutation/Rearrangement Frequencies and Methylation Patterns at a Given DNA Locus Using Restriction Fragment Length Polymorphism
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    Chapter 7 Isoschizomers and Amplified Fragment Length Polymorphism for the Detection of Specific Cytosine Methylation Changes
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    Chapter 8 Analysis of small RNA populations using hybridization to DNA tiling arrays.
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    Chapter 9 Northern Blotting Techniques for Small RNAs
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    Chapter 10 qRT-PCR of Small RNAs.
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    Chapter 11 Cloning New Small RNA Sequences
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    Chapter 12 Genome-Wide Mapping of Protein-DNA Interaction by Chromatin Immunoprecipitation and DNA Microarray Hybridization (ChIP-chip). Part A: ChIP-chip Molecular Methods
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    Chapter 13 Genome-Wide Mapping of Protein-DNA Interaction by Chromatin Immunoprecipitation and DNA Microarray Hybridization (ChIP-chip). Part B: ChIP-chip Data Analysis
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    Chapter 14 Metaanalysis of ChIP-chip Data
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    Chapter 15 Chromatin Immunoprecipitation Protocol for Histone Modifications and Protein–DNA Binding Analyses in Arabidopsis
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    Chapter 16 cDNA Libraries for Virus-Induced Gene Silencing
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    Chapter 17 Detection and Quantification of DNA Strand Breaks Using the ROPS (Random Oligonucleotide Primed Synthesis) Assay
  19. Altmetric Badge
    Chapter 18 Reporter Gene-Based Recombination Lines for Studies of Genome Stability
  20. Altmetric Badge
    Chapter 19 Plant Transgenesis
Attention for Chapter 10: qRT-PCR of Small RNAs.
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Mentioned by

wikipedia
7 Wikipedia pages

Citations

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4 Dimensions

Readers on

mendeley
60 Mendeley
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1 CiteULike
Summary Wikipedia Dimensions citations
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Chapter title
qRT-PCR of Small RNAs.
Chapter number 10
Book title
Plant Epigenetics
Published in
Methods in molecular biology, December 2009
DOI 10.1007/978-1-60761-646-7_10
Pubmed ID
20204872
Book ISBNs
978-1-60761-645-0, 978-1-60761-646-7
Authors

Varkonyi-Gasic E, Hellens RP, Erika Varkonyi-Gasic, Roger P. Hellens, Varkonyi-Gasic, Erika, Hellens, Roger P.

Abstract

Plant small RNAs are a class of 19- to 25-nucleotide (nt) RNA molecules that are essential for genome stability, development and differentiation, disease, cellular communication, signaling, and adaptive responses to biotic and abiotic stress. Small RNAs comprise two major RNA classes, short interfering RNAs (siRNAs) and microRNAs (miRNAs). Efficient and reliable detection and quantification of small RNA expression has become an essential step in understanding their roles in specific cells and tissues. Here we provide protocols for the detection of miRNAs by stem-loop RT-PCR. This method enables fast and reliable miRNA expression profiling from as little as 20 pg of total RNA extracted from plant tissue and is suitable for high-throughput miRNA expression analysis. In addition, this method can be used to detect other classes of small RNAs, provided the sequence is known and their GC contents are similar to those specific for miRNAs.

View on publisher site
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 60 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United States 1 2%
Brazil 1 2%
Unknown 58 97%

Demographic breakdown

Readers by professional status Count As %
Researcher 11 18%
Student > Master 11 18%
Student > Bachelor 8 13%
Student > Ph. D. Student 8 13%
Student > Doctoral Student 6 10%
Other 13 22%
Unknown 3 5%
Readers by discipline Count As %
Agricultural and Biological Sciences 33 55%
Biochemistry, Genetics and Molecular Biology 13 22%
Medicine and Dentistry 4 7%
Immunology and Microbiology 2 3%
Pharmacology, Toxicology and Pharmaceutical Science 1 2%
Other 2 3%
Unknown 5 8%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 3. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 15 February 2022.
All research outputs
#7,585,435
of 23,130,383 outputs
Outputs from Methods in molecular biology
#2,353
of 13,269 outputs
Outputs of similar age
#48,793
of 164,894 outputs
Outputs of similar age from Methods in molecular biology
#38
of 120 outputs
Altmetric has tracked 23,130,383 research outputs across all sources so far. This one is in the 44th percentile – i.e., 44% of other outputs scored the same or lower than it.
So far Altmetric has tracked 13,269 research outputs from this source. They receive a mean Attention Score of 3.4. This one has done well, scoring higher than 76% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 164,894 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 21st percentile – i.e., 21% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 120 others from the same source and published within six weeks on either side of this one. This one is in the 31st percentile – i.e., 31% of its contemporaries scored the same or lower than it.

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