| Chapter title |
Encapsulation of nanoparticles in virus protein shells.
|
|---|---|
| Chapter number | 1 |
| Book title |
Protein Cages
|
| Published in |
Methods in molecular biology, January 2015
|
| DOI | 10.1007/978-1-4939-2131-7_1 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-2130-0, 978-1-4939-2131-7
|
| Authors |
Irina B Tsvetkova, Bogdan G Dragnea, Irina B. Tsvetkova, Bogdan G. Dragnea |
| Abstract |
The self-assembly of virus-like particles may lead to materials which combine the unique characteristics of viruses, such as precise size control and responsivity to environmental cues, with the properties of abiotic cargo. For a few different viruses, shell proteins are amenable to the in vitro encapsulation of non-genomic cargo in a regular protein cage. In this chapter we describe protocols of high-efficiency in vitro self-assembly around functionalized gold nanoparticles for three examples of icosahedral and non-icosahedral viral protein cages derived from a plant virus, an animal virus, and a human retrovirus. These protocols can be readily adapted with small modifications to work for a broad variety of inorganic and organic nanoparticles. |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Japan | 1 | 6% |
| United States | 1 | 6% |
| Unknown | 15 | 88% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 4 | 24% |
| Student > Ph. D. Student | 3 | 18% |
| Professor > Associate Professor | 2 | 12% |
| Student > Bachelor | 2 | 12% |
| Student > Master | 2 | 12% |
| Other | 2 | 12% |
| Unknown | 2 | 12% |
| Readers by discipline | Count | As % |
|---|---|---|
| Chemistry | 4 | 24% |
| Biochemistry, Genetics and Molecular Biology | 3 | 18% |
| Pharmacology, Toxicology and Pharmaceutical Science | 1 | 6% |
| Chemical Engineering | 1 | 6% |
| Immunology and Microbiology | 1 | 6% |
| Other | 3 | 18% |
| Unknown | 4 | 24% |