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Plant DNA Fingerprinting and Barcoding

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Cover of 'Plant DNA Fingerprinting and Barcoding'

Table of Contents

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    Book Overview
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    Chapter 1 A Taxonomist’s View on Genomic Authentication
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    Chapter 2 DNA Fingerprinting, DNA Barcoding, and Next Generation Sequencing Technology in Plants
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    Chapter 3 Challenges in the DNA Barcoding of Plant Material
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    Chapter 4 Plant Genetics for Forensic Applications
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    Chapter 5 DNA Purification from Multiple Sources in Plant Research with Homemade Silica Resins
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    Chapter 6 Random Amplified Marker Technique for Plants Rich in Polyphenols
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    Chapter 7 Amplified Fragment Length Polymorphism: An Invaluable Fingerprinting Technique for Genomic, Transcriptomic, and Epigenetic Studies
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    Chapter 8 Restriction Fragment Length Polymorphism of the 5S-rRNA-NTS Region: A Rapid and Precise Method for Plant Identification
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    Chapter 9 ISSR: A Reliable and Cost-Effective Technique for Detection of DNA Polymorphism.
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    Chapter 10 Development of Sequence Characterized Amplified Region from Random Amplified Polymorphic DNA Amplicons
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    Chapter 11 Authentication of Medicinal Plants by SNP-Based Multiplex PCR
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    Chapter 12 Multiplex PCR Method to Discriminate Artemisia iwayomogi from Other Artemisia Plants
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    Chapter 13 Loop-Mediated Isothermal Amplification for the Detection of Plant Pathogens
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    Chapter 14 Genomic DNA extraction and barcoding of endophytic fungi.
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    Chapter 15 Using GenBank® for Genomic Authentication: A Tutorial
Attention for Chapter 9: ISSR: A Reliable and Cost-Effective Technique for Detection of DNA Polymorphism.
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Chapter title
ISSR: A Reliable and Cost-Effective Technique for Detection of DNA Polymorphism.
Chapter number 9
Book title
Plant DNA Fingerprinting and Barcoding
Published in
Methods in molecular biology, March 2012
DOI 10.1007/978-1-61779-609-8_9
Pubmed ID
Book ISBNs
978-1-61779-608-1, 978-1-61779-609-8
Authors

Sarwat M, Maryam Sarwat

Abstract

With the emergence of more and more molecular markers as useful tools in plethora of population genetic and phylogenetic studies, choice of marker system for a particular study has become mind boggling. These marker systems differ in their advantages and disadvantages, so it is imperative to keep in mind all the pros and cons of the technique while selecting one for the problem to be addressed.Here, we have shed light on the ISSR (intersimple sequence repeat) technique, as a marker of choice if one wants to go for properties such as reliability, simplicity, cost effectiveness, and speed, in addition to assessing genetic diversity between closely related individuals. We have outlined here the whole methodology of this technique with an example of Tribulus terrestris as case study.

Mendeley readers

The data shown below were compiled from readership statistics for 22 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 22 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 6 27%
Student > Bachelor 5 23%
Student > Master 3 14%
Student > Doctoral Student 2 9%
Student > Ph. D. Student 1 5%
Other 3 14%
Unknown 2 9%
Readers by discipline Count As %
Agricultural and Biological Sciences 10 45%
Biochemistry, Genetics and Molecular Biology 7 32%
Social Sciences 1 5%
Engineering 1 5%
Unknown 3 14%