| Chapter title |
Analysis of LINE-1 Expression in Human Pluripotent Cells.
|
|---|---|
| Chapter number | 7 |
| Book title |
Human Embryonic Stem Cells Handbook
|
| Published in |
Methods in molecular biology, March 2012
|
| DOI | 10.1007/978-1-61779-794-1_7 |
| Pubmed ID | |
| Book ISBNs |
978-1-61779-793-4, 978-1-61779-794-1
|
| Authors |
Muñoz-Lopez M, Garcia-Cañadas M, Macia A, Morell S, Garcia-Perez JL, Muñoz-Lopez, Martin, Garcia-Cañadas, Marta, Macia, Angela, Morell, Santiago, Garcia-Perez, Jose L., Martin Muñoz-Lopez, Marta Garcia-Cañadas, Angela Macia, Santiago Morell, Jose L. Garcia-Perez |
| Abstract |
Half of the human genome is composed of repeated DNA, and some types are mobile within our genome (transposons and retrotransposons). Despite their abundance, only a small fraction of them are currently active in our genome (Long Interspersed Element-1 (LINE-1), Alu, and SVA elements). LINE-1 or L1 elements are a family of active non-LTR retrotransposons, the ongoing mobilization of which still impacts our genome. As selfish DNA elements, L1 activity is more prominent in early human development, where new insertions would be transmitted to the progeny. Here, we describe the conventional methods aimed to determine the expression level of LINE-1 elements in pluripotent human cells. |
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