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In Vitro Mutagenesis Protocols

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Cover of 'In Vitro Mutagenesis Protocols'

Table of Contents

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    Book Overview
  2. Altmetric Badge
    Chapter 1 Mutagenesis Protocols in Saccharomyces cerevisiae by In Vivo Overlap Extension
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    Chapter 2 In Vitro Mutagenesis of Brucella Species
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    Chapter 3 Random Mutagenesis Strategies for Campylobacter and Helicobacter Species
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    Chapter 4 Mutagenesis of the Repeat Regions of Herpesviruses Cloned as Bacterial Artificial Chromosomes
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    Chapter 5 An Efficient Protocol for VZV BAC-Based Mutagenesis
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    Chapter 6 A Method for Rapid Genetic Integration into Plasmodium falciparum Utilizing Mycobacteriophage Bxb1 Integrase
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    Chapter 7 In Vitro Mutagenesis Protocols
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    Chapter 8 A rapid and versatile PCR-based site-directed mutagenesis protocol for generation of mutations along the entire length of a cloned cDNA.
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    Chapter 9 Rapid Sequence Scanning Mutagenesis Using In Silico Oligo Design and the Megaprimer PCR of Whole Plasmid Method (MegaWHOP)
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    Chapter 10 Insertion and Deletion Mutagenesis by Overlap Extension PCR
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    Chapter 11 In Vitro Mutagenesis Protocols
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    Chapter 12 A Modified Inverse PCR Procedure for Insertion, Deletion, or Replacement of a DNA Fragment in a Target Sequence and Its Application in the Ligand Interaction Scan Method for Generation of Ligand-Regulated Proteins
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    Chapter 13 Amplification of Orthologous Genes Using Degenerate Primers
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    Chapter 14 Computational Evaluation of Protein Stability Change upon Mutations
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    Chapter 15 Approaches for Using Animal Models to Identify Loci That Genetically Interact with Human Disease-Causing Point Mutations
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    Chapter 16 Using Peptide Loop Insertion Mutagenesis for the Evolution of Proteins
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    Chapter 17 Massive Mutagenesis ® : High-Throughput Combinatorial Site-Directed Mutagenesis
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    Chapter 18 Directed In Vitro Evolution of Reporter Genes Based on Semi-Rational Design and High-Throughput Screening
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    Chapter 19 Ribosome Display for Rapid Protein Evolution by Consecutive Rounds of Mutation and Selection
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    Chapter 20 Fine-Tuning Enzyme Activity Through Saturation Mutagenesis
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    Chapter 21 Characterization of Structural Determinants of Type 1 Corticotropin Releasing Hormone (CRH) Receptor Signalling Properties
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    Chapter 22 Site-Directed Mutagenesis for Improving Biophysical Properties of V H Domains
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    Chapter 23 Phenotype Based Functional Gene Screening Using Retrovirus-Mediated Gene Trapping in Quasi-Haploid RAW 264.7 Cells
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    Chapter 24 Site-Directed Disulfide Cross-Linking to Probe Conformational Changes of a Transporter During Its Functional Cycle: Escherichia coli AcrB Multidrug Exporter as an Example
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    Chapter 25 Site-Specific Incorporation of Extra Components into RNA by Transcription Using Unnatural Base Pair Systems
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    Chapter 26 Mutagen™: A Random Mutagenesis Method Providing a Complementary Diversity Generated by Human Error-Prone DNA Polymerases
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    Chapter 27 Random-Scanning Mutagenesis
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    Chapter 28 Easy Two-Step Method for Randomizing and Cloning Gene Fragments
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    Chapter 29 Random Mutagenesis Using a Mutator Strain
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    Chapter 30 En Passant Mutagenesis: A Two Step Markerless Red Recombination System
Attention for Chapter 29: Random Mutagenesis Using a Mutator Strain
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Chapter title
Random Mutagenesis Using a Mutator Strain
Chapter number 29
Book title
In Vitro Mutagenesis Protocols
Published in
Methods in molecular biology, January 2010
DOI 10.1007/978-1-60761-652-8_29
Pubmed ID
Book ISBNs
978-1-60761-651-1, 978-1-60761-652-8
Authors

Ghazala Muteeb, Ranjan Sen, Muteeb, Ghazala, Sen, Ranjan

X Demographics

X Demographics

The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 86 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Belgium 1 1%
Unknown 85 99%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 18 21%
Student > Master 14 16%
Student > Bachelor 12 14%
Student > Doctoral Student 10 12%
Researcher 9 10%
Other 5 6%
Unknown 18 21%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 32 37%
Agricultural and Biological Sciences 24 28%
Pharmacology, Toxicology and Pharmaceutical Science 2 2%
Immunology and Microbiology 2 2%
Medicine and Dentistry 2 2%
Other 4 5%
Unknown 20 23%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 25 August 2020.
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#20,481,952
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Outputs from Methods in molecular biology
#9,975
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#158,100
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Outputs of similar age from Methods in molecular biology
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