In Vitro Mutagenesis Protocols

Chapter 1 Mutagenesis Protocols in Saccharomyces cerevisiae by In Vivo Overlap Extension

Chapter 2 In Vitro Mutagenesis of Brucella Species

Chapter 4 Mutagenesis of the Repeat Regions of Herpesviruses Cloned as Bacterial Artificial Chromosomes

Chapter 5 An Efficient Protocol for VZV BAC-Based Mutagenesis

Chapter 6 A Method for Rapid Genetic Integration into Plasmodium falciparum Utilizing Mycobacteriophage Bxb1 Integrase

Chapter 7 In Vitro Mutagenesis Protocols

Chapter 8 A rapid and versatile PCR-based site-directed mutagenesis protocol for generation of mutations along the entire length of a cloned cDNA.

Chapter 9 Rapid Sequence Scanning Mutagenesis Using In Silico Oligo Design and the Megaprimer PCR of Whole Plasmid Method (MegaWHOP)

Chapter 10 Insertion and Deletion Mutagenesis by Overlap Extension PCR

Chapter 11 In Vitro Mutagenesis Protocols

Chapter 12 A Modified Inverse PCR Procedure for Insertion, Deletion, or Replacement of a DNA Fragment in a Target Sequence and Its Application in the Ligand Interaction Scan Method for Generation of Ligand-Regulated Proteins

Chapter 13 Amplification of Orthologous Genes Using Degenerate Primers

Chapter 14 Computational Evaluation of Protein Stability Change upon Mutations

Chapter 15 Approaches for Using Animal Models to Identify Loci That Genetically Interact with Human Disease-Causing Point Mutations

Chapter 16 Using Peptide Loop Insertion Mutagenesis for the Evolution of Proteins

Chapter 17 Massive Mutagenesis ® : High-Throughput Combinatorial Site-Directed Mutagenesis

Chapter 18 Directed In Vitro Evolution of Reporter Genes Based on Semi-Rational Design and High-Throughput Screening

Chapter 19 Ribosome Display for Rapid Protein Evolution by Consecutive Rounds of Mutation and Selection

Chapter 20 Fine-Tuning Enzyme Activity Through Saturation Mutagenesis

Chapter 21 Characterization of Structural Determinants of Type 1 Corticotropin Releasing Hormone (CRH) Receptor Signalling Properties

Chapter 22 Site-Directed Mutagenesis for Improving Biophysical Properties of V H Domains

Chapter 23 Phenotype Based Functional Gene Screening Using Retrovirus-Mediated Gene Trapping in Quasi-Haploid RAW 264.7 Cells

Chapter 24 Site-Directed Disulfide Cross-Linking to Probe Conformational Changes of a Transporter During Its Functional Cycle: Escherichia coli AcrB Multidrug Exporter as an Example

Chapter 25 Site-Specific Incorporation of Extra Components into RNA by Transcription Using Unnatural Base Pair Systems

Chapter 26 Mutagen™: A Random Mutagenesis Method Providing a Complementary Diversity Generated by Human Error-Prone DNA Polymerases

Chapter 27 Random-Scanning Mutagenesis

Chapter 28 Easy Two-Step Method for Randomizing and Cloning Gene Fragments

Chapter 29 Random Mutagenesis Using a Mutator Strain

Chapter 30 En Passant Mutagenesis: A Two Step Markerless Red Recombination System