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Hedgehog Signaling Protocols

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Cover of 'Hedgehog Signaling Protocols'

Table of Contents

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    Book Overview
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    Chapter 1 Controlled Delivery of Sonic Hedgehog with a Heparin-Based Coacervate
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    Chapter 2 In Vivo Imaging of Hedgehog Transport in Drosophila Epithelia.
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    Chapter 3 Modeling Hedgehog Signaling Through Flux-Saturated Mechanisms
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    Chapter 4 Evaluating the Activity of Smoothened Toward G Proteins Using [ 35 S]Guanosine 5′-(3- O -thio)triphosphate ([ 35 S]GTPγS)
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    Chapter 5 Analysis of Smoothened Phosphorylation and Activation in Cultured Cells and Wing Discs of Drosophila
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    Chapter 6 Hedgehog Signaling Protocols
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    Chapter 7 Luciferase Reporter Assays to Study Transcriptional Activity of Hedgehog Signaling in Normal and Cancer Cells
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    Chapter 8 Measuring Expression Levels of Endogenous Gli Genes by Immunoblotting and Real-Time PCR
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    Chapter 9 Quantitative Immunoblotting of Endogenous Hedgehog Pathway Components
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    Chapter 10 Measuring Gli2 Phosphorylation by Selected Reaction Monitoring Mass Spectrometry
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    Chapter 11 Rapid Screening of Gli2/3 Mutants Using the Flp-In System
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    Chapter 12 Insights into Gli Factors Ubiquitylation Methods
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    Chapter 13 Determination of Acetylation of the Gli Transcription Factors
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    Chapter 14 Efficient Detection of Indian Hedgehog During Endochondral Ossification by Whole-Mount Immunofluorescence
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    Chapter 15 Methods for Detection of Ptc1-Driven LacZ Expression in Adult Mouse Skin
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    Chapter 16 Determination and Analysis of Cellular Metabolic Changes by Noncanonical Hedgehog Signaling.
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Hedgehog Signaling Protocols
Published by
Springer New York, July 2015
DOI 10.1007/978-1-4939-2772-2
978-1-4939-2771-5, 978-1-4939-2772-2

Riobo, Natalia A.

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Geographical breakdown

Country Count As %
Unknown 1 100%

Demographic breakdown

Readers by professional status Count As %
Other 1 100%
Readers by discipline Count As %
Neuroscience 1 100%