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Mendeley readers
| Chapter title |
Methods for Detection of Ptc1-Driven LacZ Expression in Adult Mouse Skin
|
|---|---|
| Chapter number | 15 |
| Book title |
Hedgehog Signaling Protocols
|
| Published in |
Methods in molecular biology, January 2015
|
| DOI | 10.1007/978-1-4939-2772-2_15 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-2771-5, 978-1-4939-2772-2
|
| Authors |
Donna M. Brennan-Crispi, Mỹ G. Mahoney, Natalia A. Riobo |
| Abstract |
The Ptc-lacZ reporter mice are a highly utilized animal model for studying both normal tissue development and cancer. Identifying cell specific activation of Hedgehog (Hh) signaling is essential to understand the effects of this critical and complex signaling pathway. β-gal detection in tissues can be difficult, with various staining procedures yielding differential results. Thus, detailed information on staining protocols is essential for determining the ideal method for a given study. Furthermore, immunohistochemical staining of X-Gal stained tissues can provide further insight into other key players in Hh signaling activation. |
Mendeley readers
The data shown below were compiled from readership statistics for 2 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 2 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 1 | 50% |
| Professor > Associate Professor | 1 | 50% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 1 | 50% |
| Medicine and Dentistry | 1 | 50% |