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Hedgehog Signaling Protocols

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Cover of 'Hedgehog Signaling Protocols'

Table of Contents

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    Book Overview
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    Chapter 1 Controlled Delivery of Sonic Hedgehog with a Heparin-Based Coacervate
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    Chapter 2 In Vivo Imaging of Hedgehog Transport in Drosophila Epithelia.
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    Chapter 3 Modeling Hedgehog Signaling Through Flux-Saturated Mechanisms
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    Chapter 4 Evaluating the Activity of Smoothened Toward G Proteins Using [ 35 S]Guanosine 5′-(3- O -thio)triphosphate ([ 35 S]GTPγS)
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    Chapter 5 Analysis of Smoothened Phosphorylation and Activation in Cultured Cells and Wing Discs of Drosophila
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    Chapter 6 Hedgehog Signaling Protocols
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    Chapter 7 Luciferase Reporter Assays to Study Transcriptional Activity of Hedgehog Signaling in Normal and Cancer Cells
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    Chapter 8 Measuring Expression Levels of Endogenous Gli Genes by Immunoblotting and Real-Time PCR
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    Chapter 9 Quantitative Immunoblotting of Endogenous Hedgehog Pathway Components
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    Chapter 10 Measuring Gli2 Phosphorylation by Selected Reaction Monitoring Mass Spectrometry
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    Chapter 11 Rapid Screening of Gli2/3 Mutants Using the Flp-In System
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    Chapter 12 Insights into Gli Factors Ubiquitylation Methods
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    Chapter 13 Determination of Acetylation of the Gli Transcription Factors
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    Chapter 14 Efficient Detection of Indian Hedgehog During Endochondral Ossification by Whole-Mount Immunofluorescence
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    Chapter 15 Methods for Detection of Ptc1-Driven LacZ Expression in Adult Mouse Skin
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    Chapter 16 Determination and Analysis of Cellular Metabolic Changes by Noncanonical Hedgehog Signaling.
Attention for Chapter 3: Modeling Hedgehog Signaling Through Flux-Saturated Mechanisms
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Chapter title
Modeling Hedgehog Signaling Through Flux-Saturated Mechanisms
Chapter number 3
Book title
Hedgehog Signaling Protocols
Published in
Methods in molecular biology, January 2015
DOI 10.1007/978-1-4939-2772-2_3
Pubmed ID
Book ISBNs
978-1-4939-2771-5, 978-1-4939-2772-2
Authors

Óscar Sánchez, Juan Calvo, Carmen Ibáñez, Isabel Guerrero, Juan Soler, Sánchez, Óscar, Calvo, Juan, Ibáñez, Carmen, Guerrero, Isabel, Soler, Juan

Abstract

Hedgehog (Hh) molecules act as morphogens directing cell fate during development by activating various target genes in a concentration dependent manner. Hitherto, modeling morphogen gradient formation in multicellular systems has employed linear diffusion, which is very far from physical reality and needs to be replaced by a deeper understanding of nonlinearities. We have developed a novel mathematical approach by applying flux-limited spreading (FLS) to Hh morphogenetic actions. In the new model, the characteristic velocity of propagation of each morphogen is a new key biological parameter. Unlike in linear diffusion models, FLS modeling predicts concentration fronts and correct patterns and cellular responses over time. In addition, FLS considers not only extracellular binding partners influence, but also channels or bridges of information transfer, such as specialized filopodia or cytonemes as a mechanism of Hh transport. We detect and measure morphogen particle velocity in cytonemes in the Drosophila wing imaginal disc. Indeed, this novel approach to morphogen gradient formation can contribute to future research in the field.

Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 11 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United States 1 9%
Unknown 10 91%

Demographic breakdown

Readers by professional status Count As %
Researcher 3 27%
Student > Ph. D. Student 2 18%
Professor 1 9%
Other 1 9%
Student > Doctoral Student 1 9%
Other 1 9%
Unknown 2 18%
Readers by discipline Count As %
Environmental Science 3 27%
Engineering 2 18%
Biochemistry, Genetics and Molecular Biology 1 9%
Economics, Econometrics and Finance 1 9%
Agricultural and Biological Sciences 1 9%
Other 0 0%
Unknown 3 27%